Key features and details
- Rabbit polyclonal to IRX7
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-IRX7 antibody
DescriptionRabbit polyclonal to IRX7
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
- WB: A549 whole cell lysate; Rat heart lysate. IHC-P: Human cervical cancer tissue.
This product was previously labelled as IRX6
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab234984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500.|
|WB||1/500 - 1/5000. Predicted molecular weight: 48 kDa.|
Sequence similaritiesBelongs to the TALE/IRO homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- Homeodomain protein IRXB3 antibody
- Iroquois class Homeodomain Protein 6 antibody
- Iroquois class homeodomain protein IRX 6 antibody
All lanes : Anti-IRX7 antibody (ab234984) at 1/500 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : Rat heart lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48 kDa
Paraffin-embedded human cervical cancer tissue stained for IRX7 using ab234984 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234984 has not yet been referenced specifically in any publications.