Anti-ISCA2 antibody (ab235776)
Key features and details
- Rabbit polyclonal to ISCA2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ISCA2 antibody -
Description
Rabbit polyclonal to ISCA2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan -
Immunogen
Recombinant full length protein corresponding to Human ISCA2 aa 1-200. Full length protein without the transit protein (1-8 aa).
Database link: Q86U28 -
Positive control
- WB: HL-60 whole cell lysate. IHC-P: Human prostate cancer and tonsil tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000.
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|
IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/1000 - 1/5000. |
IHC-P
1/20 - 1/200. |
Target
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Function
Involved in the assembly of mitochondrial iron-sulfur proteins. Probably involved in the binding of an intermediate of Fe/S cluster assembly. -
Sequence similarities
Belongs to the hesB/iscA family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 122961 Human
- GenBank: NM_194279 Human
- SwissProt: Q86U28 Human
- Unigene: 291079 Human
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Alternative names
- c14_5557 antibody
- HESB like domain containing 1 antibody
- HESB like domain containing protein 1 antibody
see all
Images
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Anti-ISCA2 antibody (ab235776) at 1/1000 dilution + HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution -
Paraffin-embedded human tonsil tissue stained for ISCA2 using ab235776 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human prostate cancer tissue stained for ISCA2 using ab235776 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab235776 has not yet been referenced specifically in any publications.