• Product name

  • Description

    Rabbit polyclonal to ISCU
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-167 of Human ISCU.

  • Positive control

    • A549 and HeLa whole cell lysate (ab150035); HeLa cells; A549 xenograft tissue.



Our Abpromise guarantee covers the use of ab154060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 18 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.


  • Function

    Involved in the assembly or repair of the [Fe-S] clusters present in iron-sulfur proteins. Binds iron.
  • Tissue specificity

    Detected in heart, liver, skeletal muscle, brain, pancreas, kidney, lung and placenta.
  • Involvement in disease

    Defects in ISCU are the cause of myopathy with exercise intolerance Swedish type (MEIS) [MIM:255125]; also known as myopathy with deficiency of succinate dehydrogenase and aconitase or myoglobinuria due to abnormal glycolysis or hereditary myopathy with lactic acidosis (HML). This autosomal recessive metabolic disease is characterized by lifelong severe exercise intolerance, in which minor exertion causes fatigue of active muscles, shortness of breath, and cardiac palpitations in association with lactic acidosis. The biochemical phenotype is characterized by a deficiency in mitochondrial iron-sulfur proteins and impaired muscle oxidative metabolism.
  • Sequence similarities

    Belongs to the nifU family.
  • Cellular localization

    Cytoplasm. Nucleus and Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2310020H20Rik antibody
    • HML antibody
    • hnifU antibody
    • Iron sulfur cluster assembly enzyme ISCU mitochondrial antibody
    • Iron sulfur cluster scaffold homolog (E. coli) antibody
    • Iron sulfur cluster scaffold homolog antibody
    • Iron-sulfur cluster assembly enzyme ISCU antibody
    • Iscu antibody
    • IscU iron sulfur cluster scaffold homolog antibody
    • ISCU_HUMAN antibody
    • ISU2 antibody
    • MGC74517 antibody
    • mitochondrial antibody
    • NIFU antibody
    • NifU like N terminal domain containing antibody
    • NifU like N terminal domain containing protein antibody
    • NifU like protein antibody
    • NifU-like N-terminal domain-containing protein antibody
    • NifU-like protein antibody
    • NIFUN antibody
    • Nitrogen fixation cluster like antibody
    • OTTHUMP00000238760 antibody
    • OTTHUMP00000238761 antibody
    • OTTHUMP00000238762 antibody
    • OTTHUMP00000238764 antibody
    • OTTHUMP00000238765 antibody
    see all


  • Anti-ISCU antibody (ab154060) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 18 kDa

    12% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling ISCU with ab154060 at 1/200 dilution. Lower image co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue labeling ISCU with ab154060 at 1/500 dilution.


ab154060 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154060.
Please use the links above to contact us or submit feedback about this product.

For licensing inquiries, please contact partnerships@abcam.com

Sign up