Key features and details
- Rabbit polyclonal to ISCU
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ISCU antibody
See all ISCU primary antibodies
DescriptionRabbit polyclonal to ISCU
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment corresponding to a region within amino acids 1-167 of Human ISCU.
- A549 and HeLa whole cell lysate (ab150035); HeLa cells; A549 xenograft tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 18 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).|
|ICC/IF||1/100 - 1/1000.|
FunctionInvolved in the assembly or repair of the [Fe-S] clusters present in iron-sulfur proteins. Binds iron.
Tissue specificityDetected in heart, liver, skeletal muscle, brain, pancreas, kidney, lung and placenta.
Involvement in diseaseDefects in ISCU are the cause of myopathy with exercise intolerance Swedish type (MEIS) [MIM:255125]; also known as myopathy with deficiency of succinate dehydrogenase and aconitase or myoglobinuria due to abnormal glycolysis or hereditary myopathy with lactic acidosis (HML). This autosomal recessive metabolic disease is characterized by lifelong severe exercise intolerance, in which minor exertion causes fatigue of active muscles, shortness of breath, and cardiac palpitations in association with lactic acidosis. The biochemical phenotype is characterized by a deficiency in mitochondrial iron-sulfur proteins and impaired muscle oxidative metabolism.
Sequence similaritiesBelongs to the nifU family.
Cellular localizationCytoplasm. Nucleus and Mitochondrion.
- Information by UniProt
- 2310020H20Rik antibody
- HML antibody
- hnifU antibody
Anti-ISCU antibody (ab154060) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 18 kDa
12% SDS PAGE
Immunofluorescent analysis of methanol-fixed HeLa cells labeling ISCU with ab154060 at 1/200 dilution. Lower image co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue labeling ISCU with ab154060 at 1/500 dilution.
ab154060 has not yet been referenced specifically in any publications.