Overview

  • Product name
  • Description
    Rabbit polyclonal to ISG15
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide derived from the N terminus of Human ISG15.

  • Positive control
    • Human prostate tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab131119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB 1/100 - 1/500. Predicted molecular weight: 18 kDa.Can be blocked with ISG15 peptide (ab210532).
ELISA 1/1000.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections.
    In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy.
  • Tissue specificity
    Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.
  • Sequence similarities
    Contains 2 ubiquitin-like domains.
  • Cellular localization
    Cytoplasm. Secreted. UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • G1P2 antibody
    • hUCRP antibody
    • IFI 15 antibody
    • IFI15 antibody
    • Interferon alpha inducible protein antibody
    • Interferon induced protein 15 antibody
    • Interferon induced protein IFI-15K antibody
    • Interferon stimulated protein 15 kDa antibody
    • Interferon, alpha-inducible protein (clone IFI-15K) antibody
    • Interferon-induced 15 kDa protein antibody
    • Interferon-induced 17 kDa protein antibody
    • Interferon-induced 17-kDa/15-kDa protein antibody
    • IP17 antibody
    • ISG 15 antibody
    • ISG15 antibody
    • ISG15 ubiquitin like modifier antibody
    • ISG15_HUMAN antibody
    • Ubiquitin cross-reactive protein antibody
    • Ubiquitin-like protein ISG15 antibody
    • UCRP antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labelling ISG15 with ab131119 at 10 µg/ml, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

This product has been referenced in:
  • Chen RH  et al. ISG15 predicts poor prognosis and promotes cancer stem cell phenotype in nasopharyngeal carcinoma. Oncotarget 7:16910-22 (2016). WB, IHC . Read more (PubMed: 26919245) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spinal cord)
Specification
Spinal cord
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 22 2016

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 3% · Temperature: RT°C
Sample
Mouse Tissue sections (retina)
Specification
retina
Permeabilization
Yes - triton 0.1%
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 22 2014

Answer

Thank you for your inquiry.

I've been in touch with the supplying lab and unfortunately after they reviewed their files, they did not have any data pertaining to whether this antibody can recognize ISG15 when conjugated to other proteins.

I am sorry that this information could not be more helpful, but please contact us with any other questions.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up