Anti-ISG15 antibody (ab131119)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-ISG15 antibody
See all ISG15 primary antibodies -
DescriptionRabbit polyclonal to ISG15
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-Fr, WB, ELISA, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
KLH conjugated synthetic peptide derived from the N terminus of Human ISG15.
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Positive control
- Human prostate tissue
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
PurityProtein G purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab131119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | 1/100 - 1/500. Predicted molecular weight: 18 kDa.Can be blocked with ISG15 peptide (ab210532). | |
| ELISA | 1/1000. | |
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionUbiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections.
In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy. -
Tissue specificityDetected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.
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Sequence similaritiesContains 2 ubiquitin-like domains.
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Cellular localizationCytoplasm. Secreted. UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted.
- Information by UniProt
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Database links
- Entrez Gene: 9636 Human
- Omim: 147571 Human
- SwissProt: P05161 Human
- Unigene: 458485 Human
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Alternative names
- G1P2 antibody
- hUCRP antibody
- IFI 15 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISG15 antibody (ab131119)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labelling ISG15 with ab131119 at 10 µg/ml, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Datasheets and documents
References
This product has been referenced in:
- Chen RH et al. ISG15 predicts poor prognosis and promotes cancer stem cell phenotype in nasopharyngeal carcinoma. Oncotarget 7:16910-22 (2016). WB, IHC . Read more (PubMed: 26919245) »
- Pencik J et al. STAT3 regulated ARF expression suppresses prostate cancer metastasis. Nat Commun 6:7736 (2015). Read more (PubMed: 26198641) »
