For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    isg15-antibody-ab131119.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ub-like Proteins
Share by email

Anti-ISG15 antibody (ab131119)

  • Datasheet
Reviews (3)Q&A (1)References (4)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISG15 antibody (ab131119)

    Key features and details

    • Rabbit polyclonal to ISG15
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Primary
    Product image
    Anti-CD24 antibody [EPR3006(N)] (ab179821)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    ELISA
    Product image
    Human Neuron specific Enolase ELISA Kit (ab217778)

    View more associated products

    Overview

    • Product name

      Anti-ISG15 antibody
      See all ISG15 primary antibodies
    • Description

      Rabbit polyclonal to ISG15
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      KLH conjugated synthetic peptide derived from the N terminus of Human ISG15.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
    • Storage buffer

      Preservative: 0.09% Sodium azide
      Constituent: 99% PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteasome / Ubiquitin
      • Ub-like Proteins
      • Epigenetics and Nuclear Signaling
      • Ubiquitin & Ubiquitin Like Modifiers
      • Ubiquitin like Modifiers

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Immunizing Peptide (Blocking)

      • ISG15 peptide (ab210532)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human ISG15 protein (ab78929)

    Applications

    Our Abpromise guarantee covers the use of ab131119 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Target

    • Function

      Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections.
      In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy.
    • Tissue specificity

      Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.
    • Sequence similarities

      Contains 2 ubiquitin-like domains.
    • Cellular localization

      Cytoplasm. Secreted. UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted.
    • Target information above from: UniProt accession P05161 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9636 Human
      • Omim: 147571 Human
      • SwissProt: P05161 Human
      • Unigene: 458485 Human
      • Alternative names

        • G1P2 antibody
        • hUCRP antibody
        • IFI 15 antibody
        • IFI15 antibody
        • Interferon alpha inducible protein antibody
        • Interferon induced protein 15 antibody
        • Interferon induced protein IFI-15K antibody
        • Interferon stimulated protein 15 kDa antibody
        • Interferon, alpha-inducible protein (clone IFI-15K) antibody
        • Interferon-induced 15 kDa protein antibody
        • Interferon-induced 17 kDa protein antibody
        • Interferon-induced 17-kDa/15-kDa protein antibody
        • IP17 antibody
        • ISG 15 antibody
        • ISG15 antibody
        • ISG15 ubiquitin like modifier antibody
        • ISG15_HUMAN antibody
        • Ubiquitin cross-reactive protein antibody
        • Ubiquitin-like protein ISG15 antibody
        • UCRP antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISG15 antibody (ab131119)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISG15 antibody (ab131119)
        Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labelling ISG15 with ab131119 at 10 µg/ml, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (4)

      Publishing research using ab131119? Please let us know so that we can cite the reference in this datasheet.

      ab131119 has been referenced in 4 publications.

      • Chen B  et al. Knockdown of interferon-stimulated gene 15 affects the sensitivity of hepatocellular carcinoma cells to norcantharidin. Exp Ther Med 18:3751-3758 (2019). PubMed: 31611931
      • Zhang SH  et al. Aberrant activation of the type I interferon system may contribute to the pathogenesis of anti-melanoma differentiation-associated gene 5 dermatomyositis. Br J Dermatol 180:1090-1098 (2019). PubMed: 29947075
      • Chen RH  et al. ISG15 predicts poor prognosis and promotes cancer stem cell phenotype in nasopharyngeal carcinoma. Oncotarget 7:16910-22 (2016). WB, IHC . PubMed: 26919245
      • Pencik J  et al. STAT3 regulated ARF expression suppresses prostate cancer metastasis. Nat Commun 6:7736 (2015). PubMed: 26198641

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-4 of 4 Abreviews or Q&A

      Imaging Mass Cytometry™ abreview for Anti-ISG15 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Mass Cytometry
      Sample
      Human Tissue sections (Liver)
      Specification
      Liver
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 21 2020

      Immunohistochemistry (Frozen sections) abreview for Anti-ISG15 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Spinal cord)
      Specification
      Spinal cord
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 22 2016

      Immunohistochemistry (Frozen sections) abreview for Anti-ISG15 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Blocking step
      BSA as blocking agent for 40 minute(s) · Concentration: 3% · Temperature: RT°C
      Sample
      Mouse Tissue sections (retina)
      Specification
      retina
      Permeabilization
      Yes - triton 0.1%
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 22 2014

      Question

      I'm looking for a good ISG15 antibody for work with both mouse tissue lysates and human cell lines. I've been looking at the ab131119 antibody and just had a question about its specificity. I need the antibody to recognize ISG15 conjugates and none of the data presented with the antibody illustrate this. Therefore I was wondering whether this antibody recognizes the conjugates or simply the free ISG15.

      Read More

      Abcam community

      Verified customer

      Asked on Jul 09 2012

      Answer

      Thank you for your inquiry.

      I've been in touch with the supplying lab and unfortunately after they reviewed their files, they did not have any data pertaining to whether this antibody can recognize ISG15 when conjugated to other proteins.

      I am sorry that this information could not be more helpful, but please contact us with any other questions.

      Read More

      Abcam Scientific Support

      Answered on Jul 09 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.