Key features and details
- Rabbit polyclonal to ISG15
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ISG15 antibody
See all ISG15 primary antibodies
DescriptionRabbit polyclonal to ISG15
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
KLH conjugated synthetic peptide derived from the N terminus of Human ISG15.
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Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab131119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionUbiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections.
In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy.
Tissue specificityDetected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.
Sequence similaritiesContains 2 ubiquitin-like domains.
Cellular localizationCytoplasm. Secreted. UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted.
- Information by UniProt
- G1P2 antibody
- hUCRP antibody
- IFI 15 antibody
ab131119 has been referenced in 4 publications.
- Chen B et al. Knockdown of interferon-stimulated gene 15 affects the sensitivity of hepatocellular carcinoma cells to norcantharidin. Exp Ther Med 18:3751-3758 (2019). PubMed: 31611931
- Zhang SH et al. Aberrant activation of the type I interferon system may contribute to the pathogenesis of anti-melanoma differentiation-associated gene 5 dermatomyositis. Br J Dermatol 180:1090-1098 (2019). PubMed: 29947075
- Chen RH et al. ISG15 predicts poor prognosis and promotes cancer stem cell phenotype in nasopharyngeal carcinoma. Oncotarget 7:16910-22 (2016). WB, IHC . PubMed: 26919245
- Pencik J et al. STAT3 regulated ARF expression suppresses prostate cancer metastasis. Nat Commun 6:7736 (2015). PubMed: 26198641