• Product name

  • Description

    Rabbit polyclonal to ISG15
  • Host species

  • Tested applications

    Suitable for: WB, ELISA, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant ISG15(Human).

  • Positive control

    • Most human cell lysates can be used as a positive control without induction or stimulation.
  • General notes

    Centrifuge product if not completely clear after standing at room temperature.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process, which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit serum.
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab14374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Detects a band of approximately 18.5 kDa.
ELISA 1/2000 - 1/10000.
IHC-Fr Use at an assay dependent concentration. PubMed: 20186858


  • Function

    Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections.
    In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy.
  • Tissue specificity

    Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.
  • Sequence similarities

    Contains 2 ubiquitin-like domains.
  • Cellular localization

    Cytoplasm. Secreted. UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • G1P2 antibody
    • hUCRP antibody
    • IFI 15 antibody
    • IFI15 antibody
    • Interferon alpha inducible protein antibody
    • Interferon induced protein 15 antibody
    • Interferon induced protein IFI-15K antibody
    • Interferon stimulated protein 15 kDa antibody
    • Interferon, alpha-inducible protein (clone IFI-15K) antibody
    • Interferon-induced 15 kDa protein antibody
    • Interferon-induced 17 kDa protein antibody
    • Interferon-induced 17-kDa/15-kDa protein antibody
    • IP17 antibody
    • ISG 15 antibody
    • ISG15 antibody
    • ISG15 ubiquitin like modifier antibody
    • ISG15_HUMAN antibody
    • Ubiquitin cross-reactive protein antibody
    • Ubiquitin-like protein ISG15 antibody
    • UCRP antibody
    see all


  • Anti-ISG15 antibody (ab14374) at 1/500 dilution + MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 12 minutes

    The band observed at 16 kDa could potentially be a cleaved form of ISG15 due to the presence of an 8 amino acid propeptide.


This product has been referenced in:

  • Lee JW  et al. Single-tubule RNA-Seq uncovers signaling mechanisms that defend against hyponatremia in SIADH. Kidney Int 93:128-146 (2018). ICC/IF ; Rat . Read more (PubMed: 28843412) »
  • Dong X  et al. Triiodothyronine alleviates alcoholic liver disease injury through the negative regulation of the NLRP3 signaling pathway. Exp Ther Med 16:1866-1872 (2018). Read more (PubMed: 30186412) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Thank you for your enquiry. No, I do not know of anyone that did not see these extra bands. It is expected to see these bands. My understanding is that these extra bands come from the binding of other proteins to ISG15 and that these proteins are not separated from ISG15 during the sample preparation and running on an SDS-PAGE gel because they are coupled to ISG15. This is one antibody recognizing ISG15. I would not expect an antibody purification to change your result. The antibody will detect ISG15 in an ELISA, however, it may also detect ISG15 that is covalently linked to other proteins, conjugates, or ubiquitin-like modifiers. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

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Thank you for your phone call. Yes, ab14374 and ab14687 are the same antibody. I have removed ab14687 from our list and so you can go ahead and order ab14374. Please let me know if you have any additional questions.

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