Overview

  • Product name
  • Description
    Rabbit polyclonal to ISG15
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human ISG15 (internal sequence). The exact sequence is proprietary.
    Database link: P05161

  • Positive control
    • WB: HeLa cell extract; Rat spleen extract. IHC-P: Human gastric cancer tissue. ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 18 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections.
    In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy.
  • Tissue specificity
    Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.
  • Sequence similarities
    Contains 2 ubiquitin-like domains.
  • Cellular localization
    Cytoplasm. Secreted. UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • G1P2 antibody
    • hUCRP antibody
    • IFI 15 antibody
    • IFI15 antibody
    • Interferon alpha inducible protein antibody
    • Interferon induced protein 15 antibody
    • Interferon induced protein IFI-15K antibody
    • Interferon stimulated protein 15 kDa antibody
    • Interferon, alpha-inducible protein (clone IFI-15K) antibody
    • Interferon-induced 15 kDa protein antibody
    • Interferon-induced 17 kDa protein antibody
    • Interferon-induced 17-kDa/15-kDa protein antibody
    • IP17 antibody
    • ISG 15 antibody
    • ISG15 antibody
    • ISG15 ubiquitin like modifier antibody
    • ISG15_HUMAN antibody
    • Ubiquitin cross-reactive protein antibody
    • Ubiquitin-like protein ISG15 antibody
    • UCRP antibody
    see all

Images

  • All lanes : Anti-ISG15 antibody (ab227541) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 2 : ISG15 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 18 kDa



    15% SDS-PAGE gel.

  • Anti-ISG15 antibody (ab227541) at 1/2000 dilution + Rat spleen extract at 50 µg

    Predicted band size: 18 kDa



    15% SDS-PAGE gel.

  • Paraffin-embedded human gastric cancer tissue stained for ISG15 using ab227541 at 1/500 dilution in immunohistochemical analysis.

  • 4% paraformaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ISG15 (green) using ab227541 at 1/100 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab227541 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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