• Product name
    Anti-ISG15 antibody [EPR3446]
    See all ISG15 primary antibodies
  • Description
    Rabbit monoclonal [EPR3446] to ISG15
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues of Human ISG15 (UniProt: P05161).

  • Positive control
    • WB: Daudi whole cell lysate (ab3951), IFN treated wild type HAP1 cell lysate, HeLa (untreated) and HeLa treated with 10 ng/ml IFN- a1 for 16 hours whole cell lysates ICC/IF: HeLa cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab133346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 15 kDa (predicted molecular weight: 18 kDa).
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections.
      In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy.
    • Tissue specificity
      Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.
    • Sequence similarities
      Contains 2 ubiquitin-like domains.
    • Cellular localization
      Cytoplasm. Secreted. UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • G1P2 antibody
      • hUCRP antibody
      • IFI 15 antibody
      • IFI15 antibody
      • Interferon alpha inducible protein antibody
      • Interferon induced protein 15 antibody
      • Interferon induced protein IFI-15K antibody
      • Interferon stimulated protein 15 kDa antibody
      • Interferon, alpha-inducible protein (clone IFI-15K) antibody
      • Interferon-induced 15 kDa protein antibody
      • Interferon-induced 17 kDa protein antibody
      • Interferon-induced 17-kDa/15-kDa protein antibody
      • IP17 antibody
      • ISG 15 antibody
      • ISG15 antibody
      • ISG15 ubiquitin like modifier antibody
      • ISG15_HUMAN antibody
      • Ubiquitin cross-reactive protein antibody
      • Ubiquitin-like protein ISG15 antibody
      • UCRP antibody
      see all


    • Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: HAP1 IFN treated wild type HAP1 whole cell lysate (20 µg)
      Lane 3: ISG15 (KO) whole cell lysate (20 µg)
      Lane 4: ISG15 (KO) IFN alpha treated whole cell lysate (20 µg)
      Lane 5: Daudi whole cell lysate (20 µg)
      Lanes 1 - 5: Merged signal (red and green). Green - ab133346 observed at 18 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab133346 was shown to specifically react with HAP1 IFN treated when HAP1 IFN treated knockout samples were used. Wild-type and HAP1 IFN treated knockout samples were subjected to SDS-PAGE. Ab133346 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/30000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-ISG15 antibody [EPR3446] (ab133346) at 1/2000 dilution

      Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10 ng/ml IFN- a1 for 16 hours whole cell lysate

      Lysates/proteins at 20 µg per lane.

      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 18 kDa
      Observed band size: 17 kDa
      why is the actual band size different from the predicted?

      Exposure time: 3 minutes

      Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    • ImmunImmunofluorescent analysis of ISG15 in HeLa cells stained with ab133346 at a 1/100 dilution.


    ab133346 has not yet been referenced specifically in any publications.

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