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Rabbit polyclonal to ISL-2
Predicted to work with:
Rat, Cow, Human
Synthetic peptide corresponding to Mouse ISL-2 aa 150-250 conjugated to keyhole limpet haemocyanin.
Database link: Q9CXV0
This antibody gave a positive signal in the following Mouse tissue lysates: E10 Embryonic Brain; E11 Embryonic Brain; E12 Embryonic Brain; E16 Embryonic Brain.
Previously labelled as Islet 2.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 40 kDa).
Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways.
Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains.
Information by UniProt
Insulin gene enhancer protein ISL 2 antibody
Insulin gene enhancer protein ISL-2 antibody
Western blot - Anti-ISL-2 antibody (ab156621)
All lanes :
Anti-ISL-2 antibody (ab156621) at 1 µg/ml
Lane 1 :
E10 Mouse Embryo Brain Tissue Lysate
Lane 2 :
E11 Mouse Embryo Brain Tissue Lysate
Lane 3 :
E12 Ms Embryo Brain Tissue Lysate
Lane 4 :
E16 Ms Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG H&L (HRP) (
) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
43 kDa (
why is the actual band size different from the predicted?
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab156621 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
has not yet been referenced specifically in any publications.
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