Overview

  • Product name
  • Description
    Rabbit polyclonal to ISL-2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse ISL-2 aa 150-250 conjugated to keyhole limpet haemocyanin.
    Database link: Q9CXV0

  • Positive control
    • This antibody gave a positive signal in the following Mouse tissue lysates: E10 Embryonic Brain; E11 Embryonic Brain; E12 Embryonic Brain; E16 Embryonic Brain.
  • General notes

    Previously labelled as Islet 2.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 40 kDa).

Target

  • Function
    Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways.
  • Sequence similarities
    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ10160 antibody
    • Insulin gene enhancer protein ISL 2 antibody
    • Insulin gene enhancer protein ISL-2 antibody
    • Insulin gene enhancer protein ISL2 antibody
    • ISL 2 antibody
    • ISL 2 transcription factor antibody
    • ISL 2 transcription factor LIM/homeodomain antibody
    • ISL LIM homeobox 2 antibody
    • ISL2 antibody
    • ISL2 transcription factor antibody
    • ISL2 transcription factor LIM/homeodomain antibody
    • ISL2_HUMAN antibody
    • Islet 2 antibody
    • Islet-2 antibody
    • Islet2 antibody
    • SL2 transcription factor LIM/homeodomain (islet-2) antibody
    see all

Images

  • All lanes : Anti-ISL-2 antibody (ab156621) at 1 µg/ml

    Lane 1 : E10 Mouse Embryo Brain Tissue Lysate
    Lane 2 : E11 Mouse Embryo Brain Tissue Lysate
    Lane 3 : E12 Ms Embryo Brain Tissue Lysate
    Lane 4 : E16 Ms Embryo Brain Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 43 kDa (why is the actual band size different from the predicted?)


    Exposure time: 150 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab156621 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab156621 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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