Product nameAnti-ISL-2 antibody [EPR12785]
See all ISL-2 primary antibodies
DescriptionRabbit monoclonal [EPR12785] to ISL-2
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Predicted to work with: ZebrafishDoes not react with: Mouse, Rat
Synthetic peptide within Human ISL-2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q96A47
- 293T, HeLa and HUVEC cell line lysates; 293 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab176576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 40 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionTranscriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways.
Sequence similaritiesContains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains.
- Information by UniProt
- FLJ10160 antibody
- Insulin gene enhancer protein ISL 2 antibody
- Insulin gene enhancer protein ISL-2 antibody
All lanes : Anti-ISL-2 antibody [EPR12785] (ab176576) at 1/1000 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 40 kDa
Flow cytometric analysis of permeabilized 293 cells labeling Islet 2 with ab176576 at a 1/10 dilution (red), or a rabbit IgG negative control (green).
ab176576 has not yet been referenced specifically in any publications.