Recombinant
RabMAb

Anti-ISL-2 antibody [EPR12785] (ab176576)

Overview

  • Product name
    Anti-ISL-2 antibody [EPR12785]
    See all ISL-2 primary antibodies
  • Description
    Rabbit monoclonal [EPR12785] to ISL-2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: ZebrafishDoes not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human ISL-2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q96A47

  • Positive control
    • 293T, HeLa and HUVEC cell line lysates; 293 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12785
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways.
    • Sequence similarities
      Contains 1 homeobox DNA-binding domain.
      Contains 2 LIM zinc-binding domains.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ10160 antibody
      • Insulin gene enhancer protein ISL 2 antibody
      • Insulin gene enhancer protein ISL-2 antibody
      • Insulin gene enhancer protein ISL2 antibody
      • ISL 2 antibody
      • ISL 2 transcription factor antibody
      • ISL 2 transcription factor LIM/homeodomain antibody
      • ISL LIM homeobox 2 antibody
      • ISL2 antibody
      • ISL2 transcription factor antibody
      • ISL2 transcription factor LIM/homeodomain antibody
      • ISL2_HUMAN antibody
      • Islet 2 antibody
      • Islet-2 antibody
      • Islet2 antibody
      • SL2 transcription factor LIM/homeodomain (islet-2) antibody
      see all

    Images

    • All lanes : Anti-ISL-2 antibody [EPR12785] (ab176576) at 1/1000 dilution

      Lane 1 : HUVEC cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 40 kDa

    • Flow cytometric analysis of permeabilized 293 cells labeling Islet 2 with ab176576 at a 1/10 dilution (red), or a rabbit IgG negative control (green).

    References

    ab176576 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab176576.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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