For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    islet-1-antibody-1h9-ab86472.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Cell Type Marker Neuron marker Soma marker
Share by email
Validated using a knockout cell line

Anti-Islet 1 antibody [1H9] (ab86472)

  • Datasheet
  • SDS
Reviews (1)Q&A (3)References (12)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [1H9] (ab86472)
  • Western blot - Anti-Islet 1 antibody [1H9] (ab86472)
  • Western blot - Anti-Islet 1 antibody [1H9] (ab86472)
  • Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [1H9] (ab86472)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [1H9] (ab86472)
  • Flow Cytometry - Anti-Islet 1 antibody [1H9] (ab86472)

Key features and details

  • Mouse monoclonal [1H9] to Islet 1
  • Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

You may also be interested in

Primary
Product image
Anti-PDX1 antibody [EPR3358(2)] (ab134150)
Protein
Recombinant Human Islet 1 protein (ab134882)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-Islet 1 antibody [1H9]
    See all Islet 1 primary antibodies
  • Description

    Mouse monoclonal [1H9] to Islet 1
  • Host species

    Mouse
  • Specificity

    In western blot, we observe a specific band at ~40kDa which is not seen in KO cell lines. A cross-reactive band was observed in the wild-type and knockout cells. The additional band below this band of interest is seen at ~38kDa in both the WT and KO cells. We are unsure as to the identity of these extra bands.
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Recombinant fragment corresponding to Human Islet 1 aa 150-349. Purified recombinant fragment of Human Islet 1 expressed in E. Coli
    Database link: P61371

  • Positive control

    • Transfected HEK293 cell lysate. Human lung cancer tissue and cervical carcinoma tissue.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR141006-29 are from Tissue Culture Supernatant

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    1H9
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators
    • Stem Cells
    • Neural Stem Cells
    • Neuron Restricted Lineage
    • Developmental Biology
    • Lineage specification
    • Endoderm
    • Developmental Biology
    • Organogenesis
    • Gut development
    • Pancreas development
    • Neuroscience
    • Development

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human Islet 1 protein (ab134882)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab86472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/50 - 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
1/500 - 1/2000. Predicted molecular weight: 39 kDa.
IHC-P
1/200 - 1/1000.
ICC/IF
1/200 - 1/1000.
Notes
Flow Cyt
1/50 - 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
1/500 - 1/2000. Predicted molecular weight: 39 kDa.
IHC-P
1/200 - 1/1000.
ICC/IF
1/200 - 1/1000.

Target

  • Function

    Binds to one of the cis-acting domain of the insulin gene enhancer.
  • Tissue specificity

    Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.
  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P61371 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3670 Human
    • Omim: 600366 Human
    • SwissProt: P61371 Human
    • Unigene: 505 Human
    • Alternative names

      • Insulin gene enhancer protein ISL 1 antibody
      • Insulin gene enhancer protein ISL-1 antibody
      • Insulin related protein antibody
      • ISL 1 antibody
      • ISL LIM homeobox 1 antibody
      • ISL1 antibody
      • ISL1 transcription factor LIM homeodomain antibody
      • ISL1 transcription factor, LIM/homeodomain (islet 1) antibody
      • ISL1 transcription factor, LIM/homeodomain antibody
      • ISL1_HUMAN antibody
      • Islet-1 antibody
      • Islet1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [1H9] (ab86472)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [1H9] (ab86472)
      ab86472 at a 1/200 dilution staining Islet 1 in human lung cancer tissue. DAB staining shows nuclear localisation.
    • Western blot - Anti-Islet 1 antibody [1H9] (ab86472)
      Western blot - Anti-Islet 1 antibody [1H9] (ab86472)
      All lanes : Anti-Islet 1 antibody [1H9] (ab86472) at 1/500 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : ISL1 (Islet 1) knockout HAP1 whole cell lysate
      Lane 3 : SH-SY5Y whole cell lysate
      Lane 4 : HepG2 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 39 kDa
      Observed band size: 40 kDa why is the actual band size different from the predicted?



      Lanes 1 - 4: Merged signal (red and green). Green - ab86472 observed at 40 kDa. Red - loading control, ab176560, observed at 50 kDa.

      ab86472 was shown to recognize Islet 1 in wild-type HAP1 cells as signal was lost at the expected MW in Islet 1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Islet 1 knockout samples were subjected to SDS-PAGE. ab86472 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Islet 1 antibody [1H9] (ab86472)
      Western blot - Anti-Islet 1 antibody [1H9] (ab86472)
      Anti-Islet 1 antibody [1H9] (ab86472) at 1/500 dilution + HEK293 cells transfected with full length Islet 1 (aa1-349)-hIgGFc

      Predicted band size: 39 kDa



      Note: The molecular weight is larger than expected, as the recombinant protein is not the endogenouse protein, but rather the fc-fusion protein

    • Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [1H9] (ab86472)
      Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [1H9] (ab86472)

      Confocal immunofluorescence analysis of HEK293 cells trasfected with full-length Islet 1-hIgGFc using ab86472 at 1/150 dilution (green). Blue: DRAQ5 fluorescent DNA dye.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [1H9] (ab86472)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody [1H9] (ab86472)
      ab86472 at a 1/200 dilution staining Islet 1 in human cervical carcinoma tissue. DAB staining shows nuclear localization.
    • Flow Cytometry - Anti-Islet 1 antibody [1H9] (ab86472)
      Flow Cytometry - Anti-Islet 1 antibody [1H9] (ab86472)

      Overlay histogram showing SH-SY5Y cells stained with ab86472 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab86472, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (12)

    Publishing research using ab86472? Please let us know so that we can cite the reference in this datasheet.

    ab86472 has been referenced in 12 publications.

    • Xu J  et al. Therapeutic effects of CXCR4+ subpopulation of transgene-free induced cardiosphere-derived cells on experimental myocardial infarction. Cell Prolif 54:e13041 (2021). PubMed: 33942933
    • Akhir MKAM  et al. The Role of ISL1 and LHX5 LIM Homeobox Genes in Bladder Tumourigenesis. Malays J Med Sci 27:37-45 (2020). PubMed: 32158343
    • Park S  et al. Differentiation of Motor Neuron-Like Cells from Tonsil-Derived Mesenchymal Stem Cells and Their Possible Application to Neuromuscular Junction Formation. Int J Mol Sci 20:N/A (2019). PubMed: 31159418
    • Camberos V  et al. Effects of Spaceflight and Simulated Microgravity on YAP1 Expression in Cardiovascular Progenitors: Implications for Cell-Based Repair. Int J Mol Sci 20:N/A (2019). PubMed: 31167392
    • Hernandez I  et al. Short-term hypoxia improves early cardiac progenitor cell function in vitro. Am J Stem Cells 7:1-17 (2018). Flow Cyt ; Human . PubMed: 29531855
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

    Filter by Ratings

    Immunohistochemistry (Frozen sections) abreview for Anti-Islet 1 antibody [1H9]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Dog Tissue sections (Heart)
    Permeabilization
    Yes - 0.5% Tween80 PBS wash
    Specification
    Heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 13 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2023 Abcam plc. All rights reserved.