Anti-Islet 1 antibody (ab20670)
Key features and details
- Rabbit polyclonal to Islet 1
- Suitable for: IHC-P, ICC/IF, IHC-Fr
- Reacts with: Mouse, Rat, Human, Apteronotus leptorhynchus
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Islet 1 antibody
See all Islet 1 primary antibodies -
Description
Rabbit polyclonal to Islet 1 -
Host species
Rabbit -
Specificity
ab20670 might also detect rat and human Islet 2 protein as the immunogen used to raise this ab20670 is 86% identical to rat and human Islet 2. This has not been tested. -
Tested applications
Suitable for: IHC-P, ICC/IF, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Apteronotus leptorhynchus
Predicted to work with: Chicken, Zebrafish
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Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (3) |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| ICC/IF | (3) |
Use a concentration of 2 µg/ml.
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| IHC-Fr | (5) |
1/500.
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| Notes |
|---|
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 2 µg/ml. |
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IHC-Fr
1/500. |
Target
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Function
Binds to one of the cis-acting domain of the insulin gene enhancer. -
Tissue specificity
Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain. -
Sequence similarities
Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3670 Human
- Entrez Gene: 16392 Mouse
- Entrez Gene: 64444 Rat
- Entrez Gene: 30147 Zebrafish
- Omim: 600366 Human
- SwissProt: P61371 Human
- SwissProt: P61372 Mouse
- SwissProt: P61374 Rat
see all -
Alternative names
- Insulin gene enhancer protein ISL 1 antibody
- Insulin gene enhancer protein ISL-1 antibody
- Insulin related protein antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody (ab20670)
IHC image of Islet 1 staining in a section of formalin fixed, paraffin embedded mouse embryo E12, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval (EDTA based pH 9.0 solution, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab20670, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody (ab20670)IHC image of Islet 1 staining in a section of formalin fixed, paraffin embedded mouse embryo E12, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval (EDTA based pH 9.0 solution, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab20670, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody (ab20670)ab20670 2µg/ml staining ISLET1 in human pancreas using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear and weak cytoplasmic staining primarily in the pancreatic islet.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody (ab20670)This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, FranceDorsal root ganglion explants were dissected from 16 day-old rat embryos and cultured for 6 hours in vitro with Neurobasal Medium containing B27 supplement.
Nuclei stained positive for anti-Islet 1 antibody ab20670 at 2µg/ml. As would be expected, not all cells in this preparation were Islet 1-positive. Pre-incubation of ab20670 with the immunizing peptide ab21996 resulted in complete blocking of the antibody.
Green = ab20670
Blue = To-pro-3 nuclear stainThe level of magnification is different in each image.
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Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody (ab20670)Image from Ifkovits, Jamie L. et al. PLoS ONE 9.2 (2014): e89678. doi: 10.1371/journal.pone.0089678. Fig S3B. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunocytochemistry/ Immunofluorescence analysis of adult mouse cardiac fibroblasts labeling Islet 1 with ab20670 at 1/100 dilution (green). Samples were fixed using 4% PFA with 0.25% TritonX-100. Scale bar is 100 µM.
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Immunohistochemistry (Frozen sections) - Anti-Islet 1 antibody (ab20670)This image is courtesy of an anonymous abreview.Immunohistochemistry (Frozen sections) analysis of mouse brain tissue sections labeling Islet 1 with ab20670 at 1/200 dilution. The tissue was fixed with paraformaldehyde followed by blocking with 5% serum for 1 hour at 25°C. The tissue was incubated with ab20670 in PBST for 12 hours at 4°C. A polyclonal donkey anti-rabbit Alexa Fluor® 594 secondary antibody was used at 1/1000 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody (ab20670)This image is courtesy of an anonymous abreview.Immunocytochemistry/ Immunofluorescence analysis of mouse dorsal root ganglia (DRG) neurons labeling Islet 1 with ab20670 at 1/200 dilution. Cells were fixed with paraformaldehyde and permeabilized with Tween 20. Blocking of the cells was performed with 2% serum for 30 minutes at 21°C, followed by incubation with ab20670 for 18 hours at 4°C. A polyclonal goat anti-rabbit Alexa Fluor® 568 secondary antibody was used at 1/1000 dilution. DAPI was used to counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Islet 1 antibody (ab20670)This image is courtesy of an anonymous collaborator.Immunohistochemical analysis of paraffin-embedded mouse E11.5 somites labelling Islet 1 with ab20670 at 1/100 dilution, followed by BioGenex polymer-HRP reagent (undiluted according to manufacturer).
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Immunohistochemistry (Frozen sections) - Anti-Islet 1 antibody (ab20670)ab20670 detected Islet 1 in the nucleus of frozen sections of E11.5 mouse embryonic brain using 1 ug/ml of antibody. Brighter staining may be achieved using a greater concentration of antibody, e.g. 2.5 or 5 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (77)
ab20670 has been referenced in 77 publications.
- Ji Z et al. Activating Transcription Factor 6 Contributes to Functional Recovery After Spinal Cord Injury in Adult Zebrafish. J Mol Neurosci 71:734-745 (2021). PubMed: 32895880
- Okawa ER et al. Essential roles of insulin and IGF-1 receptors during embryonic lineage development. Mol Metab 47:101164 (2021). PubMed: 33453419
- Crane-Smith Z et al. A Highly Conserved Shh Enhancer Coordinates Hypothalamic and Craniofacial Development. Front Cell Dev Biol 9:595744 (2021). PubMed: 33869166
- He D et al. Transcription factor Isl1 is dispensable for the development of the mouse prosensory region. Cytotechnology 72:407-414 (2020). PubMed: 32219582
- Zhou X et al. Cellular and molecular properties of neural progenitors in the developing mammalian hypothalamus. Nat Commun 11:4063 (2020). PubMed: 32792525