Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Permeabilization
Yes - saponin
Specification
Human pluripotent stem cell derived cardiomyocyte
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: commercial perm/wash buffer
Dilution
1/1200
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa Fluor® 568 Goat Anti-Rabbit IgG
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 568
Additional data
Additional Notes
Antibody (red) positively stains the nuclei (blue) of cardiomyocytes (cardiac specific GFP - green) as expected. Slight background in the cytoplasm of some cells. We did expect some of the GFP negative cells to be Islet1 positive in this culture.
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Submitted Jan 12 2015