Application
Western blot
Sample
Mouse Tissue lysate - whole (Cerebellum)
Gel Running Conditions
Reduced Denaturing (4-15% gradient)
Loading amount
40 µg
Specification
Cerebellum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-Tween 0.1%
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Invitrogen, Donkey anti-Rabbit IgG (H+L) Highly Cr
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Detection
Detection method
Fluorescence (Odissey)
Exposure
7 minute(s) and 0 second(s)
Bands
Specific: 39 kDa Non-specific: 50 kDa
Additional data
Additional Notes
Predicted molecular weight: 39 kDa.
Abcam response
The customer has confirmed that an Odyssey device was used for fluorescent detection. The reason why the image is provided is in black and white is because, once it is acquired, it can be pseudo-colored in a different way, and they find that in black and white the image gives many more details compared to fluorescence with black-background.
We would like to thank the reviewer for submitting this AbReview for ab20670 and for their valuable feedback. We appreciate the time and effort that the reviewer has put into testing this antibody in western blot, an application that has not been tested before. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Jan 31 2020