Application
IHC - Wholemount
Sample
Human Embryo (Human embryonic Spinal cord)
Specification
Human embryonic Spinal cord
Other product details
Incubation time
99 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: PBS 1x + 0.5% Gelatine + 0.5% Triton
Dilution
1/10000
Secondary antibody
Dilution
1/800
Name
Abcam antibody:Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed
Additional data
Additional Notes
Incubated for 7 days at 37°C in primary antibody
1-3 days in the secondary at 37°C
then cleared using iDisco+ protocol
Imaged with light sheet microscopy
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Alain Chedotal
Verified customer
Submitted Feb 25 2021