Immunocytochemistry/ Immunofluorescence abreview for Anti-Islet 1 antibody [EP4182]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification
Human pluripotent stem cell derived cardiomyocyte
Permeabilization
Yes - saponin
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: commercial perm/wash buffer
Dilution
1/1200

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor­ 568 Goat Anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 568
Dilution
1/1000

Additional data

Additional Notes
Distinct staining of ISL1 (red) in the nuclei (blue) of cardiomyocytes (NKX2-5-GFP) derived from human pluripotent stem cells. Nuclei negative for ISL1 staining can also be observed, indicating staining is not generic nuclear staining.

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Submitted Oct 16 2014

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