ChIP abreview for Anti-Islet 1 antibody [EP4182]

Good
Abreviews
Application
ChIP
Detection step
Real-time PCR
Sample
Mouse Cell lysate - nuclear (Min6)
Specification
Min6
Negative control
Negative control for Isl-1 antibody: known locus without enrichment Positive control for IP: Antibody for another factor that is not enriched at that locus
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1.1% Formaldehyed
Positive control
Positive control for Isl-1 antibody: known locus of enrichment Positive control for IP: Antibody for another factor also enriched at that locus

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Tris-HCL buffered NaCl, SDS, Triton X100

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Submitted Mar 24 2015

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