Recombinant
RabMAb

Recombinant Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657)

Overview

  • Product name
    Anti-Islet 1 antibody [EP4182] - BSA and Azide free
    See all Islet 1 primary antibodies
  • Description
    Rabbit monoclonal [EP4182] to Islet 1 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, Flow Cyt, WB, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Chicken, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Islet 1 aa 250 to the C-terminus.
    Database link: P61371

  • Positive control
    • WB: K562, HeLa, Jurkat and SH-SY5Y cell lysates IHC-Fr: Chicken hindbrain ICC/IF: U-87 MG cell line.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    Rat: We have preliminary testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab216657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.
ICC/IF Use at an assay dependent concentration.

 

IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Binds to one of the cis-acting domain of the insulin gene enhancer.
  • Tissue specificity
    Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.
  • Sequence similarities
    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Insulin gene enhancer protein ISL 1 antibody
    • Insulin gene enhancer protein ISL-1 antibody
    • Insulin related protein antibody
    • ISL 1 antibody
    • ISL LIM homeobox 1 antibody
    • ISL1 antibody
    • ISL1 transcription factor LIM homeodomain antibody
    • ISL1 transcription factor, LIM/homeodomain (islet 1) antibody
    • ISL1 transcription factor, LIM/homeodomain antibody
    • ISL1_HUMAN antibody
    • Islet-1 antibody
    • Islet1 antibody
    see all

Images

  • For immunofluorescence, tissues were collected from mice, embedded fresh into OCT, and subsequently cut into 10μm sections using a Leica Cryostat. Embryos collected after lentiviral infection for the Shh overexpression experiment were pre-fixed for in 4% PFA 1h at RT. Slides were fixed for 10 min (or 7 min for slides of Shh overexpression embryos) in 4% PFA and blocked for 1h or overnight in PBS-Triton with BSA/NDS. Primary antibodies were diluted in blocking solution and incubations were carried out for 1h or overnight, followed by incubation in secondary antibodies for 1h at room temperature. Slides were then counterstained with DAPI and mounted using antifade mounting media.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109517).

  • Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling Islet 1 with purified ab109517 at 1/50 dilution(10 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109517).

  • ab109517 (purified) at 1/30 immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate. 10 µg of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) was used for detection at 1:1500 dilution. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109517).

  • Immunocytochemistry/Immunofluorescence analysis of SH-SY-5Y (human neuroblastoma) cells labelling Islet 1 with purified ab109517 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109517).

  • Immunocytochemistry/Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling Islet 1 with unpurified ab109517.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109517).

  • ab109517 (unpurified) immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109517).

  • IHC-Fr image of anti-Islet 1 staining with unpurified ab109517 on tissue sections from chicken hindbrain. The sections were blocked with 3% BSA for 1 hour at 4°C, before incubation with unpurified ab109517 (1/1000 dilution) for 16 hours at 4°C. The secondary was an Alexa-Fluor® 568 conjugated goat anti-rabbit polyclonal, used at a 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109517).

References

This product has been referenced in:
  • Nasif S  et al. Islet 1 specifies the identity of hypothalamic melanocortin neurons and is critical for normal food intake and adiposity in adulthood. Proc Natl Acad Sci U S A 112:E1861-70 (2015). IHC-Fr ; Mouse . Read more (PubMed: 25825735) »
  • Perdigoto CN  et al. Embryonic maturation of epidermal Merkel cells is controlled by a redundant transcription factor network. Development 141:4690-6 (2014). Read more (PubMed: 25468937) »
See all 3 Publications for this product

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