Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP4182] to Islet 1 - Neural Stem Cell Marker (Alexa Fluor® 555)
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm
Product nameAnti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (Alexa Fluor® 555)
See all Islet 1 primary antibodies
DescriptionRabbit monoclonal [EP4182] to Islet 1 - Neural Stem Cell Marker (Alexa Fluor® 555)
ConjugationAlexa Fluor® 555. Ex: 555nm, Em: 565nm
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Islet 1 aa 250 to the C-terminus.
Database link: P61371
- ICC/IF: SKNSH cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or email@example.com.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
- Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (Alexa Fluor® 594) (ab203404)
- Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (Alexa Fluor® 568) (ab203406)
- Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657)
Our Abpromise guarantee covers the use of ab203405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product gave a positive signal in SKNSH cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)
FunctionBinds to one of the cis-acting domain of the insulin gene enhancer.
Tissue specificityExpressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.
Sequence similaritiesContains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains.
- Information by UniProt
- Insulin gene enhancer protein ISL 1 antibody
- Insulin gene enhancer protein ISL-1 antibody
- Insulin related protein antibody
ab203405 staining Islet 1 in SKNSH cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203405 at a 1/166 dilution (pseudocolored in green) and ab195884, Rat monoclonal to alpha Tubulin (Alexa Fluor® 647), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in SKNSH cells fixed with 100% methanol (5 min)
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab203405 has not yet been referenced specifically in any publications.