Anti-ISLR antibody (ab232986)
Key features and details
- Rabbit polyclonal to ISLR
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-ISLR antibody -
Description
Rabbit polyclonal to ISLR -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Orangutan -
Immunogen
Recombinant full length protein corresponding to Human ISLR aa 1 to the C-terminus. Full length mature chain, expressed in E. coli.
Database link: O14498 -
Positive control
- WB: Recombinant human ISLR protein. Human serum. IHC-P: Human stomach, prostate and kidney tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 5 - 20 µg/ml.
|
|
WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 46 kDa.
|
Notes |
---|
IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 46 kDa. |
Target
-
Tissue specificity
Expressed in various tissues including retina, heart, skeletal muscle, prostate, ovary, small intestine, thyroid, adrenal cortex, testis, stomach and spinal cord. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 5 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. -
Developmental stage
Expressed in fetal lung and kidney. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 515018 Cow
- Entrez Gene: 3671 Human
- Omim: 602059 Human
- SwissProt: A4IFA6 Cow
- SwissProt: O14498 Human
- Unigene: 699822 Human
- Unigene: 710506 Human
-
Alternative names
- AW553146 antibody
- HsT17563 antibody
- Immunoglobulin superfamily containing leucine-rich repeat protein antibody
see all
Images
-
Anti-ISLR antibody (ab232986) at 2 µg/ml + Human serum
Secondary
HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 46 kDa -
Formalin-fixed, paraffin-embedded human stomach tissue stained for ISLR with ab232986 at 20 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
-
Formalin-fixed, paraffin-embedded human kidney tissue stained for ISLR with ab232986 at 20 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
-
Formalin-fixed, paraffin-embedded human prostate tissue stained for ISLR with ab232986 at 20 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
-
Anti-ISLR antibody (ab232986) at 2 µg/ml + Recombinant human ISLR protein
Secondary
HRP-Linked Guinea pig
Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 46 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab232986 has been referenced in 1 publication.
- Zhang P et al. Silencing of ISLR inhibits tumour progression and glycolysis by inactivating the IL‑6/JAK/STAT3 pathway in non‑small cell lung cancer. Int J Mol Med 48:N/A (2021). PubMed: 34713300