Key features and details
- Potent, cell-permeable MIF antagonist
- CAS Number: 478336-92-4
- Purity: > 99%
- Soluble in ethanol to 100 mM and in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Product nameISO 1, MIF-Antagonist, MIF antagonist
DescriptionPotent, cell-permeable MIF antagonist
Potent, cell-permeable MIF (migration inhibitory factor) antagonist (IC50 = 25 μM). Targets catalytic pocket of MIF. Inhibits TNF release from macrophages. Anti-inflammatory. Active in vitro and in vivo.
Chemical name4,5-Dihydro-3-(4-hydroxyphenyl)-5-isoxazoleacetic acid methyl ester
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in ethanol to 100 mM and in DMSO to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab142140 has been referenced in 2 publications.
- Ito N et al. Biphasic MIF and SDF1 expression during podocyte injury promote CD44-mediated glomerular parietal cell migration in focal segmental glomerulosclerosis. Am J Physiol Renal Physiol 318:F741-F753 (2020). PubMed: 32068458
- Adzavon YM et al. Macrophage migration inhibitory factor contributes to the pathogenesis of benign lymphoepithelial lesion of the lacrimal gland. Cell Commun Signal 16:70 (2018). PubMed: 30348174