Overview

  • Product name

    Isobavachalcone, Akt inhibitor
  • Description

    Akt inhibitor. Natural chalcone.
  • Biological description

    Inhibits Akt activation and subsequent downstream signaling to evoke apoptosis. Constituent of P. longum. Displays antiproliferative and antioxidant activity. Active in vivo.
  • Purity

    > 99%
  • CAS Number

    20784-50-3
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    (2E)-1-[2,4-Dihydroxy-3-(3-methyl-2-butenyl)phenyl]-3-(4-hydroxyphenyl)-2-propen-1-one
  • Molecular weight

    324.38
  • Molecular formula

    C20H20O4
  • PubChem identifier

    5281255
  • Storage instructions

    Store at -20°C. It is important to note that this product is reported to be light sensitive. Store In the Dark. Store under desiccating conditions.
  • Solubility overview

    Soluble in DMSO to 100 mM and ethanol to 100 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    Oc1c(ccc(O)c1C\C=C(/C)C)C(=O)/C=C/c2ccc(O)cc2
  • Source

    Psoralea corylifolia

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab141168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab7977 staining Bax in SK-N-SH cells treated with isobavachalcone (ab141168), by ICC/IF. Increase of Bax expression correlates with increased concentration of isobavachalcone, as described in literature.
    The cells were incubated at 37°C for 6h in media containing different concentrations of ab141168 (isobavachalcone) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab7977 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:

  • Jing H  et al. Abrogation of Akt signaling by Isobavachalcone contributes to its anti-proliferative effects towards human cancer cells. Cancer Lett 294:167-77 (2010). Read more (PubMed: 20167420) »
  • Ohno O  et al. Inhibitory effects of bakuchiol, bavachin, and isobavachalcone isolated from Piper longum on melanin production in B16 mouse melanoma cells. Biosci Biotechnol Biochem 74:1504-6 (2010). Read more (PubMed: 20622433) »
  • Omisore NO  et al. Antitrichomonal and antioxidant activities of Dorstenia barteri and Dorstenia convexa. Braz J Med Biol Res 38:1087-94 (2005). Read more (PubMed: 16007280) »

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab141168.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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