• Product name
    Isocitrate Dehydrogenase Assay Kit (Colorimetric)
  • Detection method
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type
    Enzyme activity
  • Sensitivity
    > 0.01 mU/well
  • Assay time
    0h 45m
  • Product overview

    Abcam's Isocitrate Dehydrogenase Assay Kit (Colorimetric) provides a convenient tool for sensitive detection of NAD(+) / NADP(+)-dependent or both IDHs in a variety of samples. The IDHs utilize isocitrate as a specific substrate leading to a proportional color development and can be easily quantified colorimetrically (λ = 450 nm) with detection sensitivity as low as 0.01 mU.
    Visit our FAQs page for tips and troubleshooting.

    Isocitrate dehydrogenase assay protocol summary:
    - add samples and standards to wells
    - incubate for 3 min and analyze with microplate reader
    - incubate for 30 min - 2 hr and analyze again

  • Platform
    Microplate reader


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Developer (lyophilized) Purple 1 vial
    IDH Assay Buffer WM 1 x 25ml
    IDH Positive Control (NADP+) Green 1 x 20µl
    IDH Substrate (lyophilized) Red 1 vial
    NAD+ (lyophilized) Blue 1 vial
    NADH Standard ( lyophilized) Yellow 1 vial
    NADP+ (lyophilized) Brown 1 vial
  • Research areas
  • Relevance
    Isocitrate dehydrogenase (IDH; EC, NAD+) is an enzyme that participates in the citric acid cycle. These IDH3 isoforms catalyze the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate and CO2 while converting NAD+ to NADH. This is a two-step process, which involves oxidation of isocitrate to oxalosuccinate, followed by the decarboxylation of the beta-carboxyl group to form the ketone, alpha-ketoglutarate. Other isoforms (EC, NADP+) catalyze the same reaction, but unrelated to the citric acid cycle. It is carried out in the mitochondrion (IDH2) as well as in the cytosol and peroxisome (IDH1) and use NADP+ as a cofactor instead of NAD+.
  • Cellular localization
    Cytoplasm. Peroxisome
  • Alternative names
    • Cytosolic NADP isocitrate dehydrogenase
    • EC=
    • IDH
    • IDP
    • Isocitrate dehydrogenase [NADP] cytoplasmic
    • NADP(+) specific ICDH
    • Oxalosuccinate decarboxylase
    • PICD
    see all


  • Isocitrate Dehydrogenase detection in Bovine Liver Extraction Sample using ab102528
  • Example of NADH and NADPH Standard Curve obtained using ab102528



This product has been referenced in:
  • Kim SR  et al. Engineered mesenchymal stem-cell-sheets patches prevents postoperative pancreatic leakage in a rat model. Sci Rep 8:360 (2018). Read more (PubMed: 29321630) »
  • López-Gallardo E  et al. Effects of Tributyltin Chloride on Cybrids with or without an ATP Synthase Pathologic Mutation. Environ Health Perspect 124:1399-405 (2016). Read more (PubMed: 27129022) »
See all 5 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Good linearity for standards and easy to use. The instructions were convoluted (and actually erroneous) but that has since been corrected. Had one issue where NADP and NAD were inversely labeled (real problem for IDH assays) but new kit was sent out without issue. Worked well on both purified enzyme and cell extracts. Unfortunately the substrate concentration is not given, so if you are interested in knowing the isocitrate concentration, you will have to make your own stock (works, we did it).

Dr. Jason Maynes

Verified customer

Submitted Apr 08 2015


Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry the leabeling issue caused some trouble and inconvenience. As requested, I have issued a free of charge replacement kit.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Thank you for bringing this to our attention. I have asked our supplying lab and our stock department to check on the kits that we currently have in stock.

The brown-capped vial should contain NADP+ and the blue-capped vial should contain NAD+. I am not sure why the blue-capped vial said NADP+. I am following up on our end to see what happened.

I apologize for the inconvenience this has caused. If you think this mislabeling affected your results, I could send you replacement kits. I will wait to hear from the lab and our stocks department to ensure that all labels are currently how they should appear.

Thanks again. Please let us know if we can be of any further assistance.

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Thank you for contacting us. You may be interested in the following kits:

Citrate Synthase: ab119692
Aconitase: ab109712 or ab83459
CitrateLyase: wedo not currently have a kit available, but weyou may be interested in ourprimary antibodies ab40793 and ab61762
NAD/NADH: ab65348 (we also havea kit forNADH Dehydrogenase: ab124535)
Isocitrate Dehydrogenase: ab102528
Isocitrate Lyase: no kits or antibodies currently available

I hope this helps, please let me know if you need any additional information or assistance.

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1. The IDH substrate is isocitrate.

2. The substrate we provide has the optimal concentration for this assay to work. However, the exact concentration is proprietary information and cannot be given out. But if you client is facing any problems in the assay which can be attributable to suboptimal concs of the substrate, please let me know and I will try to troubleshoot it.

I hope this is helpful. Please contact me again if you have any further questions.

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Thank you for contacting us and your interest in our products.

I have been checking on the suitability of the Isocitrate Dehydrogenase Colorimetric Assay Kit (ab102528)with bacterial samplesas you are hoping to use.

Although it has not been specifically tested with bacterial samples, theoretically it should work for such sample types.However, you may need to perform some optimisation, particularly with the cell numbers usedand other such parameters.The bacteria can be lysed in the assay buffer provided in this kit.

I have attached the protocol booklet of the kit for your information.

I hope I have been able to provide you with sufficient information in time for your meeting. If you have any further questions please do not hesitate to ask.

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