Overview

  • Product name
    Isocitrate Dehydrogenase Assay Kit (Colorimetric)
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type
    Enzyme activity
  • Sensitivity
    > 0.01 mU/well
  • Assay time
    0h 45m
  • Product overview

    Abcam's Isocitrate Dehydrogenase Assay Kit (Colorimetric) provides a convenient tool for sensitive detection of NAD(+) / NADP(+)-dependent or both IDHs in a variety of samples. The IDHs utilize isocitrate as a specific substrate leading to a proportional color development and can be easily quantified colorimetrically (λ = 450 nm) with detection sensitivity as low as 0.01 mU.
    Visit our FAQs page for tips and troubleshooting.


    Isocitrate dehydrogenase assay protocol summary:
    - add samples and standards to wells
    - incubate for 3 min and analyze with microplate reader
    - incubate for 30 min - 2 hr and analyze again

  • Platform
    Microplate reader

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Developer (lyophilized) Purple 1 vial
    IDH Assay Buffer WM 1 x 25ml
    IDH Positive Control (NADP+) Green 1 x 20µl
    IDH Substrate (lyophilized) Red 1 vial
    NAD+ (lyophilized) Blue 1 vial
    NADH Standard ( lyophilized) Yellow 1 vial
    NADP+ (lyophilized) Brown 1 vial
  • Research areas
  • Relevance
    Isocitrate dehydrogenase (IDH; EC 1.1.1.41, NAD+) is an enzyme that participates in the citric acid cycle. These IDH3 isoforms catalyze the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate and CO2 while converting NAD+ to NADH. This is a two-step process, which involves oxidation of isocitrate to oxalosuccinate, followed by the decarboxylation of the beta-carboxyl group to form the ketone, alpha-ketoglutarate. Other isoforms (EC 1.1.1.42, NADP+) catalyze the same reaction, but unrelated to the citric acid cycle. It is carried out in the mitochondrion (IDH2) as well as in the cytosol and peroxisome (IDH1) and use NADP+ as a cofactor instead of NAD+.
  • Cellular localization
    Cytoplasm. Peroxisome
  • Alternative names
    • Cytosolic NADP isocitrate dehydrogenase
    • EC=1.1.1.42
    • IDH
    • IDP
    • Isocitrate dehydrogenase [NADP] cytoplasmic
    • NADP(+) specific ICDH
    • Oxalosuccinate decarboxylase
    • PICD
    see all

Images

  • Isocitrate Dehydrogenase detection in Bovine Liver Extraction Sample using ab102528
  • Example of NADH and NADPH Standard Curve obtained using ab102528

Protocols

References

This product has been referenced in:
  • Kim SR  et al. Engineered mesenchymal stem-cell-sheets patches prevents postoperative pancreatic leakage in a rat model. Sci Rep 8:360 (2018). Read more (PubMed: 29321630) »
  • López-Gallardo E  et al. Effects of Tributyltin Chloride on Cybrids with or without an ATP Synthase Pathologic Mutation. Environ Health Perspect 124:1399-405 (2016). Read more (PubMed: 27129022) »
See all 4 Publications for this product

Customer reviews and Q&As

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Abreviews
Good linearity for standards and easy to use. The instructions were convoluted (and actually erroneous) but that has since been corrected. Had one issue where NADP and NAD were inversely labeled (real problem for IDH assays) but new kit was sent out without issue. Worked well on both purified enzyme and cell extracts. Unfortunately the substrate concentration is not given, so if you are interested in knowing the isocitrate concentration, you will have to make your own stock (works, we did it).

Dr. Jason Maynes

Verified customer

Submitted Apr 08 2015

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