Overview

  • Product name

    Anti-Isoleucyl tRNA synthetase antibody
    See all Isoleucyl tRNA synthetase primary antibodies
  • Description

    Rabbit polyclonal to Isoleucyl tRNA synthetase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment within Human Isoleucyl tRNA synthetase (internal sequence). The exact sequence is proprietary.
    Database link: P41252

  • Positive control

    • IHC-P: Rat brain tissue. IP: HEK-293T whole cell extract. WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab229643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/1000 - 1/10000. Predicted molecular weight: 145 kDa.
IP 1/100 - 1/500.

Target

  • Relevance

    Isoleucyl tRNA synthetase is a component of multi ARS complex and catalyzes the ligation of isoleucine with isoleucyl tRNA.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • FLJ20736 antibody
    • IARS antibody
    • IARS1 antibody
    • IleRS antibody
    • ILRS antibody
    • IRS antibody
    • Isoleucine tRNA ligase 1 cytoplasmic antibody
    • Isoleucine tRNA ligase antibody
    • Isoleucine tRNA synthetase antibody
    • Isoleucyl tRNA synthetase antibody
    • PRO0785 antibody
    see all

Images

  • All lanes : Anti-Isoleucyl tRNA synthetase antibody (ab229643) at 1/4000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 145 kDa



    5% SDS-PAGE gel.

  • Paraffin-embedded rat brain tissue stained for Isoleucyl tRNA synthetase using ab229643 at 1/500 dilution in immunohistochemical analysis.

  • Isoleucyl tRNA synthetase was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with 5 µg ab229643. Western blot was performed from the immunoprecipitate using ab229643. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: Control IgG IP in HEK-293T whole cell lysate.

    Lane 2: ab229643 IP in HEK-293T whole cell lysate.

References

This product has been referenced in:

  • Li B  et al. Up regulation of isoleucyl-tRNA synthetase promotes vascular smooth muscle cells dysfunction via p38 MAPK/PI3K signaling pathways. Life Sci 224:51-57 (2019). Read more (PubMed: 30905780) »
See 1 Publication for this product

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