Product nameAnti-Isoleucyl tRNA synthetase antibody
See all Isoleucyl tRNA synthetase primary antibodies
DescriptionRabbit polyclonal to Isoleucyl tRNA synthetase
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow
Recombinant fragment within Human Isoleucyl tRNA synthetase (internal sequence). The exact sequence is proprietary.
Database link: P41252
- IHC-P: Rat brain tissue. IP: HEK-293T whole cell extract. WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 145 kDa.|
|IP||1/100 - 1/500.|
RelevanceIsoleucyl tRNA synthetase is a component of multi ARS complex and catalyzes the ligation of isoleucine with isoleucyl tRNA.
- FLJ20736 antibody
- IARS antibody
- IARS1 antibody
All lanes : Anti-Isoleucyl tRNA synthetase antibody (ab229643) at 1/4000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 145 kDa
5% SDS-PAGE gel.
Paraffin-embedded rat brain tissue stained for Isoleucyl tRNA synthetase using ab229643 at 1/500 dilution in immunohistochemical analysis.
Isoleucyl tRNA synthetase was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with 5 µg ab229643. Western blot was performed from the immunoprecipitate using ab229643. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG IP in HEK-293T whole cell lysate.
Lane 2: ab229643 IP in HEK-293T whole cell lysate.