Key features and details
- Rabbit polyclonal to Isoleucyl tRNA synthetase
- Suitable for: IHC-P, WB, IP
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Isoleucyl tRNA synthetase antibody
See all Isoleucyl tRNA synthetase primary antibodies
DescriptionRabbit polyclonal to Isoleucyl tRNA synthetase
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow
Recombinant fragment within Human Isoleucyl tRNA synthetase (internal sequence). The exact sequence is proprietary.
Database link: P41252
- IHC-P: Rat brain tissue. IP: HEK-293T whole cell extract. WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 145 kDa.|
|IP||1/100 - 1/500.|
RelevanceIsoleucyl tRNA synthetase is a component of multi ARS complex and catalyzes the ligation of isoleucine with isoleucyl tRNA.
- FLJ20736 antibody
- IARS antibody
- IARS1 antibody
All lanes : Anti-Isoleucyl tRNA synthetase antibody (ab229643) at 1/4000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 145 kDa
5% SDS-PAGE gel.
Paraffin-embedded rat brain tissue stained for Isoleucyl tRNA synthetase using ab229643 at 1/500 dilution in immunohistochemical analysis.
Isoleucyl tRNA synthetase was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with 5 µg ab229643. Western blot was performed from the immunoprecipitate using ab229643. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG IP in HEK-293T whole cell lysate.
Lane 2: ab229643 IP in HEK-293T whole cell lysate.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229643 has been referenced in 1 publication.
- Li B et al. Up regulation of isoleucyl-tRNA synthetase promotes vascular smooth muscle cells dysfunction via p38 MAPK/PI3K signaling pathways. Life Sci 224:51-57 (2019). PubMed: 30905780