Product nameAnti-Isoleucyl tRNA synthetase antibody
See all Isoleucyl tRNA synthetase primary antibodies
DescriptionRabbit polyclonal to Isoleucyl tRNA synthetase
SpecificityThis antibody reacts specifically with Isoleucyl tRNA synthetase protein.
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
S1074-F1264 denatured recombinant human IRS.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.05% Sodium azide
Our Abpromise guarantee covers the use of ab31533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 145 kDa.|
|IP||Use at an assay dependent dilution.|
RelevanceIsoleucyl tRNA synthetase is a component of multi ARS complex and catalyzes the ligation of isoleucine with isoleucyl tRNA.
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ICC/IF image of ab31533 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31533, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-Isoleucyl tRNA synthetase antibody (ab31533) at 1/2000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml
Goat anti-rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 145 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
ab31533 has not yet been referenced specifically in any publications.