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    isoleucyl-trna-synthetase-antibody-epr10267-ab154175.pdf

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RecombinantRabMAb

Recombinant Anti-Isoleucyl tRNA synthetase antibody [EPR10267] (ab154175)

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Western blot - Anti-Isoleucyl tRNA synthetase antibody [EPR10267] (ab154175)
  • Anti-Isoleucyl tRNA synthetase antibody [EPR10267] (ab154175)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10267] to Isoleucyl tRNA synthetase
  • Suitable for: WB
  • Reacts with: Human

You may also be interested in

Protein
Product image
Recombinant Human Isoleucyl tRNA synthetase protein (ab152465)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Isoleucyl tRNA synthetase antibody [EPR10267]
    See all Isoleucyl tRNA synthetase primary antibodies
  • Description

    Rabbit monoclonal [EPR10267] to Isoleucyl tRNA synthetase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human Isoleucyl tRNA synthetase (UniProt: P41252).

  • Positive control

    • Ramos, Jurkat, HepG2 and HeLa cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10267
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNAs
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNA synthetase

Associated products

  • Alternative Versions

    • Anti-Isoleucyl tRNA synthetase antibody [EPR10267] - BSA and Azide free (ab249075)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Ramos whole cell lysate (ab3955)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human Isoleucyl tRNA synthetase protein (ab152465)

Applications

Our Abpromise guarantee covers the use of ab154175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 144 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Relevance

      Isoleucyl tRNA synthetase is a component of multi ARS complex and catalyzes the ligation of isoleucine with isoleucyl tRNA.
    • Cellular localization

      Cytoplasmic
    • Database links

      • Entrez Gene: 3376 Human
      • Omim: 600709 Human
      • SwissProt: P41252 Human
      • Alternative names

        • FLJ20736 antibody
        • IARS antibody
        • IARS1 antibody
        • IleRS antibody
        • ILRS antibody
        • IRS antibody
        • Isoleucine tRNA ligase 1 cytoplasmic antibody
        • Isoleucine tRNA ligase antibody
        • Isoleucine tRNA synthetase antibody
        • Isoleucyl tRNA synthetase antibody
        • PRO0785 antibody
        see all

      Images

      • Western blot - Anti-Isoleucyl tRNA synthetase antibody [EPR10267] (ab154175)
        Western blot - Anti-Isoleucyl tRNA synthetase antibody [EPR10267] (ab154175)
        All lanes : Anti-Isoleucyl tRNA synthetase antibody [EPR10267] (ab154175) at 1/1000 dilution

        Lane 1 : Ramos cell lysate
        Lane 2 : Jurkat cell lysate
        Lane 3 : HepG2 cell lysate
        Lane 4 : HeLa cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 144 kDa

      • Anti-Isoleucyl tRNA synthetase antibody [EPR10267] (ab154175)
        Anti-Isoleucyl tRNA synthetase antibody [EPR10267] (ab154175)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab154175? Please let us know so that we can cite the reference in this datasheet.

      ab154175 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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