Anti-Isoleucyl tRNA synthetase antibody [EPR10267] (ab154175)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Isoleucyl tRNA synthetase antibody [EPR10267]
See all Isoleucyl tRNA synthetase primary antibodies -
DescriptionRabbit monoclonal [EPR10267] to Isoleucyl tRNA synthetase
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide, corresponding to residues in Human Isoleucyl tRNA synthetase (UniProt: P41252).
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Positive control
- Ramos, Jurkat, HepG2 and HeLa cell lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20ºC.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR10267
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 144 kDa. |
Target
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RelevanceIsoleucyl tRNA synthetase is a component of multi ARS complex and catalyzes the ligation of isoleucine with isoleucyl tRNA.
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Cellular localizationCytoplasmic
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Database links
- Entrez Gene: 3376 Human
- Omim: 600709 Human
- SwissProt: P41252 Human
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Alternative names
- FLJ20736 antibody
- IARS antibody
- IARS1 antibody
see all
Images
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![Western blot - Anti-Isoleucyl tRNA synthetase antibody [EPR10267] (ab154175)](https://www.abcam.com/ps/datasheet/images/154/ab154175/Isoleucyl-tRNA-synthetase-Primary-antibodies-ab154175-1.jpg)
Western blot - Anti-Isoleucyl tRNA synthetase antibody [EPR10267] (ab154175)All lanes : Anti-Isoleucyl tRNA synthetase antibody [EPR10267] (ab154175) at 1/1000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 144 kDa
Protocols
References
ab154175 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"