Anti-Isopeptide antibody [81D1C2] (ab422)

Reviews (1)Q&A (4)References (10)

Overview

  • Product name

    Anti-Isopeptide antibody [81D1C2]

  • Description

    Mouse monoclonal [81D1C2] to Isopeptide

  • Host species

    Mouse

  • Specificity

    This antibody reacts with the isopeptide : epsilon-(gamma-glutamyl) lysine. There is no cross reaction with either lysine or glutamine. They are specific to the epsilon-(gamma-glutamyl) lysine bond formed by transglutaminase activity.

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic isopeptide.

  • General notes

    The ability of this antibody to fix complement is unknown. The isopeptide may be purchased from Bachem Catalog number G-1970.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    81D1C2

  • Myeloma

    unknown

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/500.

Target

  • Alternative names

    • Isopeptide bond antibody

References

This product has been referenced in:

  • Schopfer LM & Lockridge O Chlorpyrifos oxon promotes tubulin aggregation via isopeptide cross-linking between diethoxyphospho-Lys and Glu or Asp: Implications for neurotoxicity. J Biol Chem 293:13566-13577 (2018). Read more (PubMed: 30006344) »
  • Liu C  et al. Tissue transglutaminase contributes to the pathogenesis of preeclampsia and stabilizes placental angiotensin receptor type 1 by ubiquitination-preventing isopeptide modification. Hypertension 63:353-61 (2014). WB . Read more (PubMed: 24191290) »
See all 10 Publications for this product

Publishing research using ab422? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Q&A

Question

Customer has purchased ab422 and needs to know the following information about this antibody: how was the antigen generated? Was cross-linking (such as BSA) involved in generating the peptide? To generate a control, what should the antibody be pre-absorbed with? Any information to help her would be greatly appreciated! Thank you in advance!

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Answer

Antigen: BSA; ab422 is a monoclonal and does not need to be pre-absorbed.

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Question

Hi I`m using your ab 422. Two questions. Do you know if this antibody could be used in flow cytometry analysis? and is there a directly labeled (ex. FITC) version of this antibody? I`m also working with AB8828, is this Ab. suitable for flow studies? Thanks

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Answer

As far as we are aware, ab422 has not yet been tested in in flow cytometry. We do not stock a labelled version of this antibody. ab8828 also has not yet been tested in flow cytometry. If you do test these antibodies in FC, please let us know how you get on by submitting a summary of your results via the reviews section on the datasheets. In return we will forward a reward of your choice -- typically an Amazon gift voucher.

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Question

I am using Ab422, the antibody that recognizes N-epsilon(gamma-glutamyl)Lys. To do appropriate control I need to get N-epsilon(gamma-glutamyl)Lys itself. Do you sell this peptide? Where to get it from?

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Answer

The MAb to isopeptide shows good reactivity on cross linked protein containing isopeptide N-epsilon(gamma-glutamyl)Lys but has weak reactivity on free isopeptide. Therefore commercialy available free isopeptide from Sigma Chemical is not a good positive control.

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Question

Please explain any differences between 153-81D4 and -81D1C2. I wish to perform both western blotting and immunohistochemistry for the isopeptide.

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Answer

The difference between these two antibodies is the isotype: 153-81D4 is an IgM and 153-81D1C2 is an IgG1. They can both be used for both applications, so the choice is yours.

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