Product nameIsoprenaline hydrochloride (Isoproterenol), Non-selective beta adrenoceptor agonist
DescriptionNon-selective β adrenoceptor agonist
Biological descriptionNon-selective β adrenoceptor agonist. Structurally similar to adrenaline. Stimulates MAPK activation through cAMP dependent kinases (EC50 = 1-3 μM). Systemically active upon subcutaneous administration.
Also available in simple stock solutions (ab146724) - add 1 ml of water to get an exact, ready-to-use concentration.
Chemical name4-[1-Hydroxy-2-[(1-methylethyl)amino]ethyl]-1,2-benzenediol hydrochloride
Storage instructionsStore at Room Temperature. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab120710 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab62153 staining HSL pSer855 in NIH-3T3 cells treated with isoprenaline hydrochloride (ab120710), by ICC/IF. Increase of HSL pSer855 expression correlates with increased concentration of isoprenaline hydrochloride, as described in literature.
The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120710 (isoprenaline hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab62153 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab120710 has not yet been referenced specifically in any publications.