Isoprenaline hydrochloride (Isoproterenol), Non-selective beta adrenoceptor agonist (ab120710)

Overview

  • Product name

    Isoprenaline hydrochloride (Isoproterenol), Non-selective beta adrenoceptor agonist
  • Description

    Non-selective β adrenoceptor agonist
  • Alternative names

    • Isoproterenol
  • Biological description

    Non-selective β adrenoceptor agonist. Structurally similar to adrenaline. Stimulates MAPK activation through cAMP dependent kinases (EC50 = 1-3 μM). Systemically active upon subcutaneous administration.

    Also available in simple stock solutions (ab146724) - add 1 ml of water to get an exact, ready-to-use concentration.
  • Purity

    > 99%
  • CAS Number

    51-30-9
  • Chemical structure

    Chemical Structure

Properties

Applications

Our Abpromise guarantee covers the use of ab120710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab62153 staining HSL pSer855 in NIH-3T3 cells treated with isoprenaline hydrochloride (ab120710), by ICC/IF. Increase of HSL pSer855 expression correlates with increased concentration of isoprenaline hydrochloride, as described in literature.
    The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120710 (isoprenaline hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab62153 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:

  • Nagoor Meeran MF  et al. Preventive effects of N-acetyl cysteine on lipids, lipoproteins and myocardial infarct size in isoproterenol induced myocardial infarcted rats: an in vivo and in vitro study. Eur J Pharmacol 677:116-22 (2012). Read more (PubMed: 22178923) »
  • Brouri F  et al. Blockade of beta 1- and desensitization of beta 2-adrenoceptors reduce isoprenaline-induced cardiac fibrosis. Eur J Pharmacol 485:227-34 (2004). Read more (PubMed: 14757145) »
  • Schmitt JM & Stork PJ beta 2-adrenergic receptor activates extracellular signal-regulated kinases (ERKs) via the small G protein rap1 and the serine/threonine kinase B-Raf. J Biol Chem 275:25342-50 (2000). Read more (PubMed: 10840035) »

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