The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa. Tested in Western blot with recombinant protein, not yet shown to detect endogenous IST1.
Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvment in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells.
Belongs to the IST1 family.
Cytoplasmic vesicle. Localizes to the midbody of dividing cells.