Product nameAnti-IST1 antibody
See all IST1 primary antibodies
DescriptionRabbit polyclonal to IST1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human IST1 aa 125-317 (internal sequence). The exact sequence is proprietary.
CAKYSK EYGKLCRTNQ IGTVNDRLMH KLSVEAPPKI LVERYLIEIA KNYNVPYEPD SVVMAEAPPG VETDLIDVGF TDDVKKGGPG RGGSGGFTAP VGGPDGTVPM PMPMPMPSAN TPFSYPLPKG PSDFNGLPMG TYQAFPNIHP PQIPATPPSY ESVDDINADK NISSAQIVGP GPKPEASAKL PSRPADN
Database link: P53990
- Human spleen tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab174735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 40 kDa. Tested in Western blot with recombinant protein, not yet shown to detect endogenous IST1.|
FunctionProposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvment in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells.
Sequence similaritiesBelongs to the IST1 family.
Cellular localizationCytoplasmic vesicle. Localizes to the midbody of dividing cells.
- Information by UniProt
- hIST1 antibody
- Increased sodium tolerance 1 antibody
- Increased sodium tolerance 1 homolog antibody
ab174735 has not yet been referenced specifically in any publications.