Overview

  • Product name
  • Description
    Rabbit polyclonal to IST1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human IST1 aa 125-317 (internal sequence). The exact sequence is proprietary.
    Sequence:

    CAKYSK EYGKLCRTNQ IGTVNDRLMH KLSVEAPPKI LVERYLIEIA KNYNVPYEPD SVVMAEAPPG VETDLIDVGF TDDVKKGGPG RGGSGGFTAP VGGPDGTVPM PMPMPMPSAN TPFSYPLPKG PSDFNGLPMG TYQAFPNIHP PQIPATPPSY ESVDDINADK NISSAQIVGP GPKPEASAKL PSRPADN


    Database link: P53990

  • Positive control
    • Human spleen tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 40% Glycerol, 59% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa. Tested in Western blot with recombinant protein, not yet shown to detect endogenous IST1.

Target

  • Function
    Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvment in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells.
  • Sequence similarities
    Belongs to the IST1 family.
  • Cellular localization
    Cytoplasmic vesicle. Localizes to the midbody of dividing cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • hIST1 antibody
    • Increased sodium tolerance 1 antibody
    • Increased sodium tolerance 1 homolog antibody
    • IST1 homolog antibody
    • IST1_HUMAN antibody
    • KIAA0174 antibody
    • MAPK activating protein PM28 antibody
    • MGC117220 antibody
    • MGC128433 antibody
    • Putative MAPK-activating protein PM28 antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed,  paraffin embedded Human spleen tissue labeling IST1 with ab174735 at 10 µg/ml.

References

ab174735 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab174735.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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