Key features and details
- Rabbit polyclonal to ISY1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ISY1 antibody
See all ISY1 primary antibodies
DescriptionRabbit polyclonal to ISY1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to amino acids 23-146 of Human ISY1 (Q9ULR0).
- Human cell line U-2 OS; RT-4 cells, U-251 MG cells and Human tonsil lysates; Human stomach tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121250 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.|
FunctionMay play a role in pre-mRNA splicing.
Sequence similaritiesBelongs to the ISY1 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- FSAP33 antibody
- ISY1 antibody
- ISY1 splicing factor homolog (S. cerevisiae) antibody
All lanes : Anti-ISY1 antibody (ab121250) at 1/250 dilution
Lane 1 : Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2 : RT-4 cell lysate
Lane 3 : U-251 MG cell lysate
Lane 4 : Human plasma lysate
Lane 5 : Liver lysate
Lane 6 : Tonsil lysate
ICC/IF (Immunocytochemistry/immunofluorescence) of Human cell line U-2 OS labelling ISY1 (green) with ab121250 at 1µg/ml. Cells were fixed with PFA and permeabilized with Triton X-100. Immunofluorescent staining shows positivity in nucleus but not nucleoli.
Immunohistochemical staining of paraffin embedded Human stomach, using 1/75 ab121250, shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of Human stomach shows moderate nuclear positivity in glandular cells using ab121250
ab121250 has been referenced in 1 publication.
- Guilgur LG et al. Requirement for highly efficient pre-mRNA splicing during Drosophila early embryonic development. Elife 3:e02181 (2014). WB . PubMed: 24755291