Key features and details
- Goat polyclonal to ITGA11
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99.48% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab114113 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab114113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 133 kDa).
1 hour primary incubation is recommended for this product.
FunctionIntegrin alpha-11/beta-1 is a receptor for collagen.
Tissue specificityAccording to PubMed:10464311, highest levels of expression in uterus and heart, intermediate levels in skeletal muscle and intermediate to low levels in pancreas, kidney and placenta. According to PubMed:10486209, also found in brain, colon, lung, small intestine, stomach, testis, salivary glands, thyroid glands and prostate. Very low levels in peripheral blood lymphocytes, fetal brain and fetal liver.
Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
Developmental stageStrongly up-regulated in differentiating fetal muscle cells (in vitro).
DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
- Information by UniProt
- integrin a11 antibody
- Integrin alpha 11 antibody
- Integrin alpha-11 antibody
Anti-ITGA11 antibody (ab114113) at 1 µg/ml + Mouse skeletal muscle lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 133 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Primary incubation: 1 hour
ab114113 has been referenced in 2 publications.
- Marmotti A et al. Allogeneic Umbilical Cord-Derived Mesenchymal Stem Cells as a Potential Source for Cartilage and Bone Regeneration: AnIn VitroStudy. Stem Cells Int 2017:1732094 (2017). PubMed: 29358953
- Verma R et al. Integrin Ligation Results in Nephrin Tyrosine Phosphorylation In Vitro. PLoS One 11:e0148906 (2016). PubMed: 26848974