Key features and details
- Rabbit polyclonal to ITGA7
- Suitable for: WB, ELISA, IHC-P, IHC-Fr
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ITGA7 antibody
See all ITGA7 primary antibodies
DescriptionRabbit polyclonal to ITGA7
Specificityab75224 detects endogenous levels of a fragment of activated ITGA7 resulting from cleavage adjacent to Glu959.
Tested applicationsSuitable for: WB, ELISA, IHC-P, IHC-Frmore details
Species reactivityReacts with: Human
Synthetic peptide derived from human ITGA7
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab75224 affinity purified from rabbit antiserum by affinity chromatography usingan epitope specific immunogen.
Our Abpromise guarantee covers the use of ab75224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 129 kDa. for the full length protein.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||1/100. (see Abreview)|
FunctionIntegrin alpha-7/beta-1 is the primary laminin receptor on skeletal myoblasts and adult myofibers. During myogenic differentiation, it may induce changes in the shape and mobility of myoblasts, and facilitate their localization at laminin-rich sites of secondary fiber formation. It is involved in the maintenance of the myofibers cytoarchitecture as well as for their anchorage, viability and functional integrity. Isoform Alpha-7X2B and isoform Alpha-7X1B promote myoblast migration on laminin 1 and laminin 2/4, but isoform Alpha-7X1B is less active on laminin 1 (In vitro). Acts as Schwann cell receptor for laminin-2. Acts as a receptor of COMP and mediates its effect on vascular smooth muscle cells (VSMCs) maturation (By similarity). Required to promote contractile phenotype acquisition in differentiated airway smooth muscle (ASM) cells.
Tissue specificityIsoforms containing segment A are predominantly expressed in skeletal muscle. Isoforms containing segment B are abundantly expressed in skeletal muscle, moderately in cardiac muscle, small intestine, colon, ovary and prostate and weakly in lung and testes. Isoforms containing segment X2D are expressed at low levels in fetal and adult skeletal muscle and in cardiac muscle, but are not detected in myoblasts and myotubes. In muscle fibers isoforms containing segment A and B are expressed at myotendinous and neuromuscular junctions; isoforms containing segment C are expressed at neuromuscular junctions and at extrasynaptic sites. Isoforms containing segments X1 or X2 or, at low levels, X1X2 are expressed in fetal and adult skeletal muscle (myoblasts and myotubes) and cardiac muscle.
Involvement in diseaseDefects in ITGA7 are the cause of muscular dystrophy congenital due to integrin alpha-7 deficiency (MDCI) [MIM:613204]. A form of congenital muscular dystrophy. Patients present at birth, or within the first few months of life, with hypotonia, muscle weakness and often with joint contractures.
Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Developmental stageIn renewing intestinal epithelium, expression of isoforms containing segment B correlates with the onset of enterocytic differentiation.
modificationsADP-ribosylated on at least two sites of the extracellular domain in skeletal myotubes.
A 70 kDa form is created by proteolytic cleavage. Cleavage is elevated during myogenic differentiation and the cleaved form enhances cell adhesion and spreading on laminin.
- Information by UniProt
- alpha 7 antibody
- FLJ25220 antibody
- Integrin alpha 7 antibody
All lanes : Anti-ITGA7 antibody (ab75224) at 1/500 dilution
Lane 1 : COS-7 cells,
treated with etoposide (25 µM, 1 hour)
Lane 2 : COS-7 cells,
treated with etoposide (25 µM, 1 hour) with Cl-peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 129 kDa
Additional bands at: 26 kDa (possible cleavage fragment)
ab75224 staining ITGA7 in Human skeletal muscle tissue by Immunohistochemistry (Frozen sections). The sections were permeabilized in Triton X-100 prior to blocking with 10% Goat serum for 1 hour at 25°C. The primary antibody was diluted 1/100 in 1xPBS/10% Goat Serum/0.1% Triton and incubated with the sample for 12 hours at 4°C. An Alexa Fluor 568®-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/250.
ab75224 has been referenced in 3 publications.
- Ahmed M et al. Laminin a2 controls mouse and human stem cell behaviour during midbrain dopaminergic neuron development. Development 146:N/A (2019). PubMed: 31371375
- Simon L et al. Chronic binge alcohol consumption alters myogenic gene expression and reduces in vitro myogenic differentiation potential of myoblasts from rhesus macaques. Am J Physiol Regul Integr Comp Physiol 306:R837-44 (2014). PubMed: 24671243
- Heller KN et al. AAV-mediated overexpression of human a7 integrin leads to histological and functional improvement in dystrophic mice. Mol Ther 21:520-5 (2013). Human . PubMed: 23319059