Anti-ITGA7 antibody (ab75224)
Key features and details
- Rabbit polyclonal to ITGA7
- Suitable for: ELISA, WB, IHC-Fr, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-ITGA7 antibody
See all ITGA7 primary antibodies -
Description
Rabbit polyclonal to ITGA7 -
Host species
Rabbit -
Specificity
ab75224 detects endogenous levels of a fragment of activated ITGA7 resulting from cleavage adjacent to Glu959. -
Tested applications
Suitable for: ELISA, WB, IHC-Fr, IHC-Pmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Synthetic peptide derived from human ITGA7
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab75224 affinity purified from rabbit antiserum by affinity chromatography usingan epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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|
WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 129 kDa. for the full length protein.
|
IHC-Fr | (1) |
Use at an assay dependent concentration.
See Abreview. |
IHC-P |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Predicted molecular weight: 129 kDa. for the full length protein. |
IHC-Fr
Use at an assay dependent concentration. See Abreview. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Integrin alpha-7/beta-1 is the primary laminin receptor on skeletal myoblasts and adult myofibers. During myogenic differentiation, it may induce changes in the shape and mobility of myoblasts, and facilitate their localization at laminin-rich sites of secondary fiber formation. It is involved in the maintenance of the myofibers cytoarchitecture as well as for their anchorage, viability and functional integrity. Isoform Alpha-7X2B and isoform Alpha-7X1B promote myoblast migration on laminin 1 and laminin 2/4, but isoform Alpha-7X1B is less active on laminin 1 (In vitro). Acts as Schwann cell receptor for laminin-2. Acts as a receptor of COMP and mediates its effect on vascular smooth muscle cells (VSMCs) maturation (By similarity). Required to promote contractile phenotype acquisition in differentiated airway smooth muscle (ASM) cells. -
Tissue specificity
Isoforms containing segment A are predominantly expressed in skeletal muscle. Isoforms containing segment B are abundantly expressed in skeletal muscle, moderately in cardiac muscle, small intestine, colon, ovary and prostate and weakly in lung and testes. Isoforms containing segment X2D are expressed at low levels in fetal and adult skeletal muscle and in cardiac muscle, but are not detected in myoblasts and myotubes. In muscle fibers isoforms containing segment A and B are expressed at myotendinous and neuromuscular junctions; isoforms containing segment C are expressed at neuromuscular junctions and at extrasynaptic sites. Isoforms containing segments X1 or X2 or, at low levels, X1X2 are expressed in fetal and adult skeletal muscle (myoblasts and myotubes) and cardiac muscle. -
Involvement in disease
Defects in ITGA7 are the cause of muscular dystrophy congenital due to integrin alpha-7 deficiency (MDCI) [MIM:613204]. A form of congenital muscular dystrophy. Patients present at birth, or within the first few months of life, with hypotonia, muscle weakness and often with joint contractures. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Developmental stage
In renewing intestinal epithelium, expression of isoforms containing segment B correlates with the onset of enterocytic differentiation. -
Post-translational
modificationsADP-ribosylated on at least two sites of the extracellular domain in skeletal myotubes.
A 70 kDa form is created by proteolytic cleavage. Cleavage is elevated during myogenic differentiation and the cleaved form enhances cell adhesion and spreading on laminin. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3679 Human
- Omim: 600536 Human
- SwissProt: Q13683 Human
- Unigene: 524484 Human
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Alternative names
- alpha 7 antibody
- FLJ25220 antibody
- Integrin alpha 7 antibody
see all
Images
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All lanes : Anti-ITGA7 antibody (ab75224) at 1/500 dilution
Lane 1 : COS-7 cells,
treated with etoposide (25 µM, 1 hour)
Lane 2 : COS-7 cells,
treated with etoposide (25 µM, 1 hour) with Cl-peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 129 kDa
Additional bands at: 26 kDa (possible cleavage fragment)
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab75224 has been referenced in 5 publications.
- Bugiardini E et al. Integrin α7 Mutations Are Associated With Adult-Onset Cardiac Dysfunction in Humans and Mice. J Am Heart Assoc 11:e026494 (2022). PubMed: 36444867
- Gwili N et al. Transcriptome profiles of stem-like cells from primary breast cancers allow identification of ITGA7 as a predictive marker of chemotherapy response. Br J Cancer 125:983-993 (2021). PubMed: 34253873
- Ahmed M et al. Laminin a2 controls mouse and human stem cell behaviour during midbrain dopaminergic neuron development. Development 146:N/A (2019). PubMed: 31371375
- Simon L et al. Chronic binge alcohol consumption alters myogenic gene expression and reduces in vitro myogenic differentiation potential of myoblasts from rhesus macaques. Am J Physiol Regul Integr Comp Physiol 306:R837-44 (2014). PubMed: 24671243
- Heller KN et al. AAV-mediated overexpression of human a7 integrin leads to histological and functional improvement in dystrophic mice. Mol Ther 21:520-5 (2013). Human . PubMed: 23319059