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  1. Link

    itgb6-antibody-epr15740-ab187155.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Beta
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RecombinantRabMAb

Recombinant Anti-ITGB6 antibody [EPR15740] (ab187155)

  • Datasheet
  • Certificate of Compliance
Reviews (4) Submit a question References (2)

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Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)
  • Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)
  • Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)
  • Immunoprecipitation - Anti-ITGB6 antibody [EPR15740] (ab187155)
  • Anti-ITGB6 antibody [EPR15740] (ab187155)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15740] to ITGB6
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-ITGB6 antibody [EPR15740]
    See all ITGB6 primary antibodies
  • Description

    Rabbit monoclonal [EPR15740] to ITGB6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal skin and fetal kidney lysate. 293 and A549 cell lysate. C6, Raw264.7, PC12 and NIH3T3 cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15740
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Beta

Associated products

  • Alternative Versions

    • Anti-ITGB6 antibody [EPR15740] - BSA and Azide free (ab240302)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • NIH/3T3 whole cell lysate (ab7179)
    • RAW 264.7 whole cell lysate (ab7187)
    • A549 whole cell lysate (ab7910)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab187155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/10000 - 1/50000. Predicted molecular weight: 86 kDa.
IP
1/30 - 1/50.
Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 86 kDa.
IP
1/30 - 1/50.

Target

  • Function

    Integrin alpha-V/beta-6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion.
  • Sequence similarities

    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P18564 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3694 Human
    • Entrez Gene: 16420 Mouse
    • Entrez Gene: 311061 Rat
    • Omim: 147558 Human
    • SwissProt: P18564 Human
    • SwissProt: Q9Z0T9 Mouse
    • SwissProt: Q6AYF4 Rat
    • Unigene: 470399 Human
    • Unigene: 98193 Mouse
    • Unigene: 19828 Rat
    see all
  • Alternative names

    • Integrin beta-6 antibody
    • ITB6_HUMAN antibody
    • ITGB6 antibody

Images

  • Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)
    Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)
    All lanes : Anti-ITGB6 antibody [EPR15740] (ab187155) at 1/10000 dilution

    Lane 1 : Human fetal skin lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 86 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)
    Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)
    All lanes : Anti-ITGB6 antibody [EPR15740] (ab187155) at 1/10000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 86 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)
    Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)
    All lanes : Anti-ITGB6 antibody [EPR15740] (ab187155) at 1/10000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw264.7 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 86 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunoprecipitation - Anti-ITGB6 antibody [EPR15740] (ab187155)
    Immunoprecipitation - Anti-ITGB6 antibody [EPR15740] (ab187155)

    Lysate from 293 cells was immunoprecipitated with ab187155 at a 1/50 dilution. For the subsequent blot, ab187155 used at a 1/10000 dilution with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.

  • Anti-ITGB6 antibody [EPR15740] (ab187155)
    Anti-ITGB6 antibody [EPR15740] (ab187155)

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (2)

Publishing research using ab187155? Please let us know so that we can cite the reference in this datasheet.

ab187155 has been referenced in 2 publications.

  • Jiang K  et al. Exosome-derived ENO1 regulates integrin a6ß4 expression and promotes hepatocellular carcinoma growth and metastasis. Cell Death Dis 11:972 (2020). PubMed: 33184263
  • Sahu S  et al. InsP7 is a small-molecule regulator of NUDT3-mediated mRNA decapping and processing-body dynamics. Proc Natl Acad Sci U S A 117:19245-19253 (2020). PubMed: 32727897

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-ITGB6 antibody [EPR15740]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
10 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Jan 10 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-ITGB6 antibody [EPR15740]

Good
Abreviews
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (cardiomyocytes)
Permeabilization
Yes - 0.3% tritonX-100
Specification
cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

Submitted Sep 29 2021

Immunohistochemistry (Frozen sections) abreview for Anti-ITGB6 antibody [EPR15740]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - 0.2 % triton-X in PBS
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative
Paraformaldehyde
Read More

DR. Camilla Schinner

Verified customer

Submitted Aug 20 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ITGB6 antibody [EPR15740]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Oral mucosa)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA-pH9.0
Permeabilization
No
Specification
Oral mucosa
Blocking step
3%BSA, 10%Serum, in 1xPBST as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Tian Liang

Verified customer

Submitted Mar 03 2020

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