Recombinant
RabMAb

Recombinant Anti-ITGB6 antibody [EPR15740] (ab187155)

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Overview

  • Product name

    Anti-ITGB6 antibody [EPR15740]
    See all ITGB6 primary antibodies

  • Description

    Rabbit monoclonal [EPR15740] to ITGB6

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human ITGB6 aa 100-200. The exact sequence is proprietary.
    Database link: P18564

  • Positive control

    • Human fetal skin and fetal kidney lysate. 293 and A549 cell lysate. C6, Raw264.7, PC12 and NIH3T3 cell lysate.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15740

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 86 kDa.
IP 1/30 - 1/50.

Target

Images

  • Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)
    Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)
    All lanes : Anti-ITGB6 antibody [EPR15740] (ab187155) at 1/10000 dilution

    Lane 1 : Human fetal skin lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 86 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)
    Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)
    All lanes : Anti-ITGB6 antibody [EPR15740] (ab187155) at 1/10000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 86 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)
    Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)
    All lanes : Anti-ITGB6 antibody [EPR15740] (ab187155) at 1/10000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw264.7 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 86 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunoprecipitation - Anti-ITGB6 antibody [EPR15740] (ab187155)
    Immunoprecipitation - Anti-ITGB6 antibody [EPR15740] (ab187155)

    Lysate from 293 cells was immunoprecipitated with ab187155 at a 1/50 dilution. For the subsequent blot, ab187155 used at a 1/10000 dilution with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.

References

ab187155 has not yet been referenced specifically in any publications.

Publishing research using ab187155? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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