Recombinant Anti-ITGB6 antibody [EPR15740] (ab187155)
![Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)](https://www.abcam.com/ps/products/187/ab187155/Images/ab187155-220319-anti-itgb6-antibody-epr15740-western-blot.jpg "Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15740] to ITGB6
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ITGB6 antibody [EPR15740]
See all ITGB6 primary antibodies -
Description
Rabbit monoclonal [EPR15740] to ITGB6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal skin and fetal kidney lysate. 293 and A549 cell lysate. C6, Raw264.7, PC12 and NIH3T3 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15740 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 86 kDa.
|
| IP |
1/30 - 1/50.
|
| Notes |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 86 kDa. |
|
IP
1/30 - 1/50. |
Target
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Function
Integrin alpha-V/beta-6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. -
Sequence similarities
Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3694 Human
- Entrez Gene: 16420 Mouse
- Entrez Gene: 311061 Rat
- Omim: 147558 Human
- SwissProt: P18564 Human
- SwissProt: Q9Z0T9 Mouse
- SwissProt: Q6AYF4 Rat
- Unigene: 470399 Human
see all -
Alternative names
- Integrin beta-6 antibody
- ITB6_HUMAN antibody
- ITGB6 antibody
Images
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Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)All lanes : Anti-ITGB6 antibody [EPR15740] (ab187155) at 1/10000 dilution
Lane 1 : Human fetal skin lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 86 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
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![Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)](https://www.abcam.com/ps/products/187/ab187155/Images/ab187155-220318-anti-itgb6-antibody-epr15740-western-blot.jpg)
Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)All lanes : Anti-ITGB6 antibody [EPR15740] (ab187155) at 1/10000 dilution
Lane 1 : 293 cell lysate
Lane 2 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 86 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
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![Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)](https://www.abcam.com/ps/products/187/ab187155/Images/ab187155-220317-anti-itgb6-antibody-epr15740-western-blot.jpg)
Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)All lanes : Anti-ITGB6 antibody [EPR15740] (ab187155) at 1/10000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 86 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
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![Immunoprecipitation - Anti-ITGB6 antibody [EPR15740] (ab187155)](https://www.abcam.com/ps/products/187/ab187155/Images/ab187155-220316-anti-itgb6-antibody-epr15740-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ITGB6 antibody [EPR15740] (ab187155)Lysate from 293 cells was immunoprecipitated with ab187155 at a 1/50 dilution. For the subsequent blot, ab187155 used at a 1/10000 dilution with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.
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![Anti-ITGB6 antibody [EPR15740] (ab187155)](https://www.abcam.com/ps/products/187/ab187155/Images/ab187155-5-benefits-of-recombinant-antibodies.png)
Anti-ITGB6 antibody [EPR15740] (ab187155)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (2)
ab187155 has been referenced in 2 publications.
- Jiang K et al. Exosome-derived ENO1 regulates integrin a6ß4 expression and promotes hepatocellular carcinoma growth and metastasis. Cell Death Dis 11:972 (2020). PubMed: 33184263
- Sahu S et al. InsP7 is a small-molecule regulator of NUDT3-mediated mRNA decapping and processing-body dynamics. Proc Natl Acad Sci U S A 117:19245-19253 (2020). PubMed: 32727897
