Recombinant
RabMAb

Recombinant Anti-ITGB6 antibody [EPR15740] (ab187155)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15740] to ITGB6
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ITGB6 antibody [EPR15740]
    See all ITGB6 primary antibodies

  • Description

    Rabbit monoclonal [EPR15740] to ITGB6

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human ITGB6 aa 100-200. The exact sequence is proprietary.
    Database link: P18564

  • Positive control

    • Human fetal skin and fetal kidney lysate. 293 and A549 cell lysate. C6, Raw264.7, PC12 and NIH3T3 cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15740

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 86 kDa.
IP 1/30 - 1/50.

Target

Images

  • Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)
    Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)
    All lanes : Anti-ITGB6 antibody [EPR15740] (ab187155) at 1/10000 dilution

    Lane 1 : Human fetal skin lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 86 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)
    Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)
    All lanes : Anti-ITGB6 antibody [EPR15740] (ab187155) at 1/10000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 86 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)
    Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)
    All lanes : Anti-ITGB6 antibody [EPR15740] (ab187155) at 1/10000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw264.7 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 86 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunoprecipitation - Anti-ITGB6 antibody [EPR15740] (ab187155)
    Immunoprecipitation - Anti-ITGB6 antibody [EPR15740] (ab187155)

    Lysate from 293 cells was immunoprecipitated with ab187155 at a 1/50 dilution. For the subsequent blot, ab187155 used at a 1/10000 dilution with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.

References (0)

Publishing research using ab187155? Please let us know so that we can cite the reference in this datasheet.

ab187155 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ITGB6 antibody [EPR15740]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Oral mucosa)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA-pH9.0
Permeabilization
No
Specification
Oral mucosa
Blocking step
3%BSA, 10%Serum, in 1xPBST as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
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Tian Liang

Verified customer

Submitted Mar 03 2020

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