Anti-ITGB6 antibody [EPR15740] (ab187155)
- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-ITGB6 antibody [EPR15740]
See all ITGB6 primary antibodies -
DescriptionRabbit monoclonal [EPR15740] to ITGB6
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, IPmore details
-
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide within Human ITGB6 aa 100-200. The exact sequence is proprietary.
Database link: P18564 -
Positive control
- Human fetal skin and fetal kidney lysate. 293 and A549 cell lysate. C6, Raw264.7, PC12 and NIH3T3 cell lysate.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
-
ClonalityMonoclonal
-
Clone numberEPR15740
-
IsotypeIgG
-
Research areas
Associated products
-
Positive Controls
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab187155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 86 kDa. | |
| IP | 1/30 - 1/50. |
Target
-
FunctionIntegrin alpha-V/beta-6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion.
-
Sequence similaritiesBelongs to the integrin beta chain family.
Contains 1 VWFA domain. -
Cellular localizationMembrane.
- Information by UniProt
-
Database links
- Entrez Gene: 3694 Human
- Entrez Gene: 16420 Mouse
- Entrez Gene: 311061 Rat
- Omim: 147558 Human
- SwissProt: P18564 Human
- SwissProt: Q9Z0T9 Mouse
- SwissProt: Q6AYF4 Rat
- Unigene: 470399 Human
see all -
Alternative names
- Integrin beta-6 antibody
- ITB6_HUMAN antibody
- ITGB6 antibody
Images
-
![Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)](https://www.abcam.com/ps/products/187/ab187155/Images/ab187155-220319-ab1871551.jpg)
Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)All lanes : Anti-ITGB6 antibody [EPR15740] (ab187155) at 1/10000 dilution
Lane 1 : Human fetal skin lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 86 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
-
![Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)](https://www.abcam.com/ps/products/187/ab187155/Images/ab187155-220318-ab1871552.jpg)
Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)All lanes : Anti-ITGB6 antibody [EPR15740] (ab187155) at 1/10000 dilution
Lane 1 : 293 cell lysate
Lane 2 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 86 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
-
![Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)](https://www.abcam.com/ps/products/187/ab187155/Images/ab187155-220317-ab1871553.jpg)
Western blot - Anti-ITGB6 antibody [EPR15740] (ab187155)All lanes : Anti-ITGB6 antibody [EPR15740] (ab187155) at 1/10000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 86 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
-
![Immunoprecipitation - Anti-ITGB6 antibody [EPR15740] (ab187155)](https://www.abcam.com/ps/products/187/ab187155/Images/ab187155-220316-ab1871554.jpg)
Immunoprecipitation - Anti-ITGB6 antibody [EPR15740] (ab187155)Lysate from 293 cells was immunoprecipitated with ab187155 at a 1/50 dilution. For the subsequent blot, ab187155 used at a 1/10000 dilution with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.
References
ab187155 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"