Overview

  • Product name

    Anti-ITIH1/SHAP antibody [EPR17138-29]
    See all ITIH1/SHAP primary antibodies
  • Description

    Rabbit monoclonal [EPR17138-29] to ITIH1/SHAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human ITIH1/SHAP aa 450-700. The exact sequence is proprietary.
    Database link: P19827

  • Positive control

    • Human fetal heart and fetal kidney lysates; Human brain, plasma and serum lysates.
  • General notes

     

     

    Previously labelled as ITIH1

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17138-29
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab204513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 80, 125, 250 kDa (predicted molecular weight: 101, 86 kDa).

Target

  • Function

    May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.
    Contains a potential peptide which could stimulate a broad spectrum of phagocytotic cells.
  • Sequence similarities

    Belongs to the ITIH family.
    Contains 1 VIT domain.
    Contains 1 VWFA domain.
  • Post-translational
    modifications

    Heavy chains are linked to bikunin via chondroitin 4-sulfate esterified to the alpha-carboxyl of the C-terminal aspartate after propeptide cleavage.
    The S-linked glycan is composed of two 6-carbon sugars, possibly Glc or Gal.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • H1P antibody
    • IATIH antibody
    • IGHEP1 antibody
    • Inter alpha (globulin) inhibitor H1 antibody
    • Inter alpha (globulin) inhibitor H1 polypeptide antibody
    • Inter alpha trypsin inhibitor heavy chain 1 antibody
    • Inter alpha trypsin inhibitor heavy chain H1 antibody
    • Inter-alpha-inhibitor heavy chain 1 antibody
    • Inter-alpha-trypsin inhibitor complex component III antibody
    • Inter-alpha-trypsin inhibitor heavy chain H1 antibody
    • ITI heavy chain H1 antibody
    • ITI-HC1 antibody
    • ITIH antibody
    • ITIH1 antibody
    • ITIH1_HUMAN antibody
    • Serum-derived hyaluronan-associated protein antibody
    • SHAP antibody
    see all

Images

  • All lanes : Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 101, 86 kDa
    Observed band size: 125,250,80 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513) at 1/10000 dilution + Human brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 101, 86 kDa
    Observed band size: 125,250,80 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513) at 1/20000 dilution + Human plasma lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 101, 86 kDa
    Observed band size: 125,250,80 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513) at 1/50000 dilution + Human serum lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 101, 86 kDa
    Observed band size: 125,250,80 kDa why is the actual band size different from the predicted?


    Exposure time: 2 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

References

This product has been referenced in:

  • Smith SM & Melrose J A Retrospective Analysis of the Cartilage Kunitz Protease Inhibitory Proteins Identifies These as Members of the Inter-a-Trypsin Inhibitor Superfamily with Potential Roles in the Protection of the Articulatory Surface. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30678366) »
See 1 Publication for this product

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