Overview

  • Product name

  • Description

    Goat polyclonal to ITIH4
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide:

    ERLCVDPRHRQGPVN

    , corresponding to amino acids 744-758 of Human ITIH4 (NP_002209.2.).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab92338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May be involved in acute phase reactions.
  • Tissue specificity

    Liver specific.
  • Sequence similarities

    Belongs to the ITIH family.
    Contains 1 VIT domain.
    Contains 1 VWFA domain.
  • Post-translational
    modifications

    Cleaved by plasma kallikrein to yield 100 kDa and 35 kDa fragments, and the resulting 100 kDa fragment is further converted to a 70 kDa fragment.
    Appears to be both N- and O-glycosylated.
    The O-linked polysaccharides on Ser-696, Thr-701 and Ser-702 are probably the mucin type linked to GalNAc.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • 35 kDa inter-alpha-trypsin inhibitor heavy chain H4 antibody
    • gp120 antibody
    • H4P antibody
    • IHRP antibody
    • Inter-alpha-inhibitor heavy chain 4 antibody
    • Inter-alpha-trypsin inhibitor family heavy chain-related protein antibody
    • Inter-alpha-trypsin inhibitor heavy chain H4 antibody
    • inter-alpha-trypsin inhibitor, heavy chain-like, 1 antibody
    • ITI heavy chain H4 antibody
    • ITI-HC4 antibody
    • ITIH4 antibody
    • ITIH4_HUMAN antibody
    • ITIHL1 antibody
    • OTTHUMP00000197120 antibody
    • OTTHUMP00000197121 antibody
    • OTTHUMP00000213834 antibody
    • OTTHUMP00000213869 antibody
    • PK-120 antibody
    • PK120 antibody
    • Plasma kallikrein sensitive glycoprotein 120 antibody
    • PRO1851 antibody
    see all

Images

  • ab92338 (3.8µg/ml) staining of paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatocytes. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References

This product has been referenced in:

  • Patil K  et al. Quantitative mass spectrometric analysis to unravel glycoproteomic signature of follicular fluid in women with polycystic ovary syndrome. PLoS One 14:e0214742 (2019). Read more (PubMed: 30946770) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.

Yes the anti-ITIH4 antibody ab92338 has been tested on endogenous protein, on human liver in IHC-P and on human vitreous in Western Blot, see the Abreview we received: https://www.abcam.com/ITIH4-antibody-ab92338-reviews.html.
ab92338 should detect the target protein in serum if this protein is present in this fluid.
Unfortunately we don't have any publication for any of the anti-ITIH4 antibodies we have in our catalog (https://www.abcam.com/index.html?t=244659&pt=1).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Application
Western blot
Sample
Human Tissue lysate - other (Vitreous)
Loading amount
2 µg
Specification
Vitreous
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Oct 06 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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