Overview

  • Product name

  • Description

    Goat polyclonal to ITIH4
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide:

    ERLCVDPRHRQGPVN

    , corresponding to amino acids 744-758 of Human ITIH4 (NP_002209.2.).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab92338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May be involved in acute phase reactions.
  • Tissue specificity

    Liver specific.
  • Sequence similarities

    Belongs to the ITIH family.
    Contains 1 VIT domain.
    Contains 1 VWFA domain.
  • Post-translational
    modifications

    Cleaved by plasma kallikrein to yield 100 kDa and 35 kDa fragments, and the resulting 100 kDa fragment is further converted to a 70 kDa fragment.
    Appears to be both N- and O-glycosylated.
    The O-linked polysaccharides on Ser-696, Thr-701 and Ser-702 are probably the mucin type linked to GalNAc.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • 35 kDa inter-alpha-trypsin inhibitor heavy chain H4 antibody
    • gp120 antibody
    • H4P antibody
    • IHRP antibody
    • Inter-alpha-inhibitor heavy chain 4 antibody
    • Inter-alpha-trypsin inhibitor family heavy chain-related protein antibody
    • Inter-alpha-trypsin inhibitor heavy chain H4 antibody
    • inter-alpha-trypsin inhibitor, heavy chain-like, 1 antibody
    • ITI heavy chain H4 antibody
    • ITI-HC4 antibody
    • ITIH4 antibody
    • ITIH4_HUMAN antibody
    • ITIHL1 antibody
    • OTTHUMP00000197120 antibody
    • OTTHUMP00000197121 antibody
    • OTTHUMP00000213834 antibody
    • OTTHUMP00000213869 antibody
    • PK-120 antibody
    • PK120 antibody
    • Plasma kallikrein sensitive glycoprotein 120 antibody
    • PRO1851 antibody
    see all

Images

  • ab92338 (3.8µg/ml) staining of paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatocytes. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References

This product has been referenced in:

  • Patil K  et al. Quantitative mass spectrometric analysis to unravel glycoproteomic signature of follicular fluid in women with polycystic ovary syndrome. PLoS One 14:e0214742 (2019). Read more (PubMed: 30946770) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

Yes the anti-ITIH4 antibody ab92338 has been tested on endogenous protein, on human liver in IHC-P and on human vitreous in Western Blot, see the Abreview we received: https://www.abcam.com/ITIH4-antibody-ab92338-reviews.html.
ab92338 should detect the target protein in serum if this protein is present in this fluid.
Unfortunately we don't have any publication for any of the anti-ITIH4 antibodies we have in our catalog (https://www.abcam.com/index.html?t=244659&pt=1).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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