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    itih4-antibody-epr112332-ab180139.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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RecombinantRabMAb

Recombinant Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (1)

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Western blot - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
  • Immunoprecipitation - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
  • Western blot - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
  • Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11233(2)] to ITIH4
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-ITIH4 antibody [EPR11233(2)]
    See all ITIH4 primary antibodies
  • Description

    Rabbit monoclonal [EPR11233(2)] to ITIH4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human ITIH4 aa 1-150. The exact sequence is proprietary.
    Database link: Q14624

  • Positive control

    • Human and mouse plasma, Mouse and rat serum, Human liver and placenta tissue.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11233(2)
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cardiovascular
    • Blood
    • Serum Proteins

Associated products

  • Alternative Versions

    • Anti-ITIH4 antibody [EPR11233(2)] - BSA and Azide free (ab240221)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/60.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/5000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa).
Notes
IP
1/60.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/5000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa).

Target

  • Function

    May be involved in acute phase reactions.
  • Tissue specificity

    Liver specific.
  • Sequence similarities

    Belongs to the ITIH family.
    Contains 1 VIT domain.
    Contains 1 VWFA domain.
  • Post-translational
    modifications

    Cleaved by plasma kallikrein to yield 100 kDa and 35 kDa fragments, and the resulting 100 kDa fragment is further converted to a 70 kDa fragment.
    Appears to be both N- and O-glycosylated.
    The O-linked polysaccharides on Ser-696, Thr-701 and Ser-702 are probably the mucin type linked to GalNAc.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q14624 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3700 Human
    • Entrez Gene: 16427 Mouse
    • Entrez Gene: 54404 Rat
    • NCBI: NP_061216.2 Mouse
    • Omim: 600564 Human
    • SwissProt: Q14624 Human
    • SwissProt: Q9DBK8 Mouse
    • Unigene: 709406 Human
    • Unigene: 34819 Mouse
    • Unigene: 11308 Rat
    see all
  • Alternative names

    • 35 kDa inter-alpha-trypsin inhibitor heavy chain H4 antibody
    • gp120 antibody
    • H4P antibody
    • IHRP antibody
    • Inter-alpha-inhibitor heavy chain 4 antibody
    • Inter-alpha-trypsin inhibitor family heavy chain-related protein antibody
    • Inter-alpha-trypsin inhibitor heavy chain H4 antibody
    • inter-alpha-trypsin inhibitor, heavy chain-like, 1 antibody
    • ITI heavy chain H4 antibody
    • ITI-HC4 antibody
    • ITIH4 antibody
    • ITIH4_HUMAN antibody
    • ITIHL1 antibody
    • OTTHUMP00000197120 antibody
    • OTTHUMP00000197121 antibody
    • OTTHUMP00000213834 antibody
    • OTTHUMP00000213869 antibody
    • PK-120 antibody
    • PK120 antibody
    • Plasma kallikrein sensitive glycoprotein 120 antibody
    • PRO1851 antibody
    see all

Images

  • Western blot - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
    Western blot - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
    All lanes : Anti-ITIH4 antibody [EPR11233(2)] (ab180139) at 1/5000 dilution

    Lane 1 : Mouse serum
    Lane 2 : Mouse plasma
    Lane 3 : Rat serum

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1000 µg

    Predicted band size: 103 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5%NFDM /TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling ITIH4 with ab180139 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
    Immunoprecipitation - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

    Western blot analysis of ITIH4 in immunoprecipitation pellets from Human plasma lysate. ab180139 used at 1/60 dilution for immunoprecipitation. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000. Blocking buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ITIH4 with ab180139 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
    Western blot - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
    Anti-ITIH4 antibody [EPR11233(2)] (ab180139) at 1/5000 dilution + Human plasma at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP) at 1/1000 dilution

    Predicted band size: 103 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5%NFDM /TBST

  • Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
    Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (1)

Publishing research using ab180139? Please let us know so that we can cite the reference in this datasheet.

ab180139 has been referenced in 1 publication.

  • Kumagai T  et al. Serum IgM Glycosylation Associated with Tuberculosis Infection in Mice. mSphere 4:N/A (2019). PubMed: 30918063

Customer reviews and Q&As

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