Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-ITIH4 antibody [EPR11233(2)]
See all ITIH4 primary antibodies -
DescriptionRabbit monoclonal [EPR11233(2)] to ITIH4
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Host speciesRabbit
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Tested applicationsSuitable for: IP, IHC-P, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant fragment within Human ITIH4 aa 1-150. The exact sequence is proprietary.
Database link: Q14624 -
Positive control
- Human and mouse plasma, Mouse and rat serum, Human liver and placenta tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR11233(2)
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab180139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/60. | |
| IHC-P | 1/250 - 1/500. | |
| WB | 1/1000 - 1/5000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa). |
Target
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FunctionMay be involved in acute phase reactions.
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Tissue specificityLiver specific.
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Sequence similaritiesBelongs to the ITIH family.
Contains 1 VIT domain.
Contains 1 VWFA domain. -
Post-translational
modificationsCleaved by plasma kallikrein to yield 100 kDa and 35 kDa fragments, and the resulting 100 kDa fragment is further converted to a 70 kDa fragment.
Appears to be both N- and O-glycosylated.
The O-linked polysaccharides on Ser-696, Thr-701 and Ser-702 are probably the mucin type linked to GalNAc. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 3700 Human
- Entrez Gene: 16427 Mouse
- Entrez Gene: 54404 Rat
- NCBI: NP_061216.2 Mouse
- Omim: 600564 Human
- SwissProt: Q14624 Human
- SwissProt: Q9DBK8 Mouse
- Unigene: 709406 Human
see all -
Alternative names
- 35 kDa inter-alpha-trypsin inhibitor heavy chain H4 antibody
- gp120 antibody
- H4P antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)](https://www.abcam.com/ps/products/180/ab180139/Images/ab180139-210011-ab1801394.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling ITIH4 with ab180139 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.
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![Immunoprecipitation - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)](https://www.abcam.com/ps/products/180/ab180139/Images/ab180139-210012-ab1801395.jpg)
Immunoprecipitation - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)Western blot analysis of ITIH4 in immunoprecipitation pellets from Human plasma lysate. ab180139 used at 1/60 dilution for immunoprecipitation. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000. Blocking buffer and concentration: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)](https://www.abcam.com/ps/products/180/ab180139/Images/ab180139-210009-ab1801393.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ITIH4 with ab180139 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.
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![Western blot - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)](https://www.abcam.com/ps/products/180/ab180139/Images/ab180139-210008-ab1801392.jpg)
Western blot - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)All lanes : Anti-ITIH4 antibody [EPR11233(2)] (ab180139) at 1/5000 dilution
Lane 1 : Mouse serum
Lane 2 : Mouse plasma
Lane 3 : Rat serum
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1000 µg
Predicted band size: 103 kDa
Observed band size: 120 kDa (why is the actual band size different from the predicted?)Blocking buffer and concentration: 5%NFDM /TBST
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![Western blot - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)](https://www.abcam.com/ps/products/180/ab180139/Images/ab180139-210007-ab180139.jpg)
Western blot - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)Anti-ITIH4 antibody [EPR11233(2)] (ab180139) at 1/5000 dilution + Human plasma at 10 µg
Secondary
Anti-Rabbit IgG (HRP) at 1/1000 dilution
Predicted band size: 103 kDa
Observed band size: 120 kDa (why is the actual band size different from the predicted?)Blocking buffer and concentration: 5%NFDM /TBST
References
ab180139 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"