Overview

  • Product name

  • Description

    Goat polyclonal to ITK/EMT
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Cow, Human
  • Immunogen

    Synthetic peptide corresponding to Human ITK/EMT aa 596-607 (C terminal) (Cysteine residue). NP_005537.3.
    Sequence:

    KERPEDRPAFSR


    Database link: Q08881

  • Positive control

    • Rat thymus tissue lysate
  • General notes

    Previously labelled as ITK. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 72 kDa.

Target

  • Function

    Plays a role in T-cell proliferation and differentiation.
  • Tissue specificity

    T-cell lines and natural killer cell lines.
  • Involvement in disease

    Defects in ITK are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1) [MIM:613011]. LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
    Contains 1 Btk-type zinc finger.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Cellular localization

    Cell membrane. Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • EMT antibody
    • Homolog of mouse T cell itk/tsk antibody
    • IL 2 inducible T cell kinase antibody
    • IL2 inducible T cell kinase antibody
    • Interleukin 2 inducible T cell kinase antibody
    • Itk antibody
    • ITK_HUMAN antibody
    • Kinase EMT antibody
    • LPFS1 antibody
    • LYK antibody
    • MGC126257 antibody
    • MGC126258 antibody
    • PSCTK 2 antibody
    • PSCTK2 antibody
    • T cell specific kinase antibody
    • T-cell-specific kinase antibody
    • TSK antibody
    • Tyrosine protein kinase antibody
    • Tyrosine protein kinase ITK/TSK antibody
    • Tyrosine protein kinase Lyk antibody
    • Tyrosine-protein kinase ITK/TSK antibody
    • Tyrosine-protein kinase Lyk antibody
    see all

Images

  • Anti-ITK/EMT antibody (ab180225) at 0.1 µg + Rat Thymus lysate (in RIPA buffer) at 35 µg

    Predicted band size: 72 kDa



    Detected by chemiluminescence.

References

ab180225 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab180225.
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