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  1. Link

    itkemt-antibody-ab245629.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Kinases Tec Family
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Anti-ITK/EMT antibody (ab245629)

  • Datasheet
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  • Western blot - Anti-ITK/EMT antibody (ab245629)

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Primary

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Protein

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-ITK/EMT antibody
    See all ITK/EMT primary antibodies
  • Description

    Rabbit polyclonal to ITK/EMT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human ITK/EMT aa 1-50. The exact sequence is proprietary. NP_005537.3
    Database link: Q08881

  • Positive control

    • WB: Jurkat whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245629 was affinity purified using an epitope specific to ITK/EMT immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Tec Family

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Positive Controls

    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant human ITK/EMT protein (ab60300)
  • Related Products

    • Recombinant human ITK/EMT protein (Active) (ab204156)

Applications

Our Abpromise guarantee covers the use of ab245629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Predicted molecular weight: 72 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Plays a role in T-cell proliferation and differentiation.
    • Tissue specificity

      T-cell lines and natural killer cell lines.
    • Involvement in disease

      Defects in ITK are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1) [MIM:613011]. LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
      Contains 1 Btk-type zinc finger.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • Cellular localization

      Cell membrane. Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells.
    • Target information above from: UniProt accession Q08881 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3702 Human
      • Entrez Gene: 16428 Mouse
      • Omim: 186973 Human
      • SwissProt: Q08881 Human
      • SwissProt: Q03526 Mouse
      • Unigene: 558348 Human
      • Unigene: 339927 Mouse
      • Alternative names

        • EMT antibody
        • Homolog of mouse T cell itk/tsk antibody
        • IL 2 inducible T cell kinase antibody
        • IL2 inducible T cell kinase antibody
        • Interleukin 2 inducible T cell kinase antibody
        • Itk antibody
        • ITK_HUMAN antibody
        • Kinase EMT antibody
        • LPFS1 antibody
        • LYK antibody
        • MGC126257 antibody
        • MGC126258 antibody
        • PSCTK 2 antibody
        • PSCTK2 antibody
        • T cell specific kinase antibody
        • T-cell-specific kinase antibody
        • TSK antibody
        • Tyrosine protein kinase antibody
        • Tyrosine protein kinase ITK/TSK antibody
        • Tyrosine protein kinase Lyk antibody
        • Tyrosine-protein kinase ITK/TSK antibody
        • Tyrosine-protein kinase Lyk antibody
        see all

      Images

      • Western blot - Anti-ITK/EMT antibody (ab245629)
        Western blot - Anti-ITK/EMT antibody (ab245629)
        All lanes : Anti-ITK/EMT antibody (ab245629) at 1 µg/ml

        Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
        Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
        Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

        Lysates/proteins at 50 µg per lane.

        Developed using the ECL technique.

        Predicted band size: 72 kDa


        Exposure time: 30 seconds


        Prepared using RIPA buffer.

      Datasheets and documents

      • Datasheet
    • References

      ab245629 has not yet been referenced specifically in any publications.

      Publishing research using ab245629? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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