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Signal Transduction Protein Phosphorylation Tyrosine Kinases Tec Family
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RecombinantRabMAb

Recombinant Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)

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Western blot - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)
  • Flow Cytometry - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)
  • Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y401] to ITK/EMT - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-ITK/EMT antibody [Y401] - BSA and Azide free
    See all ITK/EMT primary antibodies
  • Description

    Rabbit monoclonal [Y401] to ITK/EMT - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab157243 is the carrier-free version of ab32039 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab157243 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    Y401
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Tec Family

Associated products

  • Alternative Versions

    • Anti-ITK/EMT antibody [Y401] (ab32039)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant human ITK/EMT protein (ab60300)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab157243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 72 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 72 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Plays a role in T-cell proliferation and differentiation.
    • Tissue specificity

      T-cell lines and natural killer cell lines.
    • Involvement in disease

      Defects in ITK are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1) [MIM:613011]. LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
      Contains 1 Btk-type zinc finger.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • Cellular localization

      Cell membrane. Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells.
    • Target information above from: UniProt accession Q08881 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3702 Human
      • Omim: 186973 Human
      • SwissProt: Q08881 Human
      • Unigene: 558348 Human
      • Alternative names

        • EMT antibody
        • Homolog of mouse T cell itk/tsk antibody
        • IL 2 inducible T cell kinase antibody
        • IL2 inducible T cell kinase antibody
        • Interleukin 2 inducible T cell kinase antibody
        • Itk antibody
        • ITK_HUMAN antibody
        • Kinase EMT antibody
        • LPFS1 antibody
        • LYK antibody
        • MGC126257 antibody
        • MGC126258 antibody
        • PSCTK 2 antibody
        • PSCTK2 antibody
        • T cell specific kinase antibody
        • T-cell-specific kinase antibody
        • TSK antibody
        • Tyrosine protein kinase antibody
        • Tyrosine protein kinase ITK/TSK antibody
        • Tyrosine protein kinase Lyk antibody
        • Tyrosine-protein kinase ITK/TSK antibody
        • Tyrosine-protein kinase Lyk antibody
        see all

      Images

      • Western blot - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)
        Western blot - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)
        Anti-ITK/EMT antibody [Y401] (ab32039) at 1/5000 dilution + Jurkat cell lysate

        Predicted band size: 72 kDa
        Observed band size: 68 kDa why is the actual band size different from the predicted?



        This data was developed using ab32039, the same antibody clone in a different buffer formulation.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)
        This data was developed using ab32039, the same antibody clone in a different buffer formulation.ab32039 at a 1/250 dilution, staining ITK/EMT in paraffin embedded human lymph node tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Flow Cytometry - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)
        Flow Cytometry - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)
        This data was developed using ab32039, the same antibody clone in a different buffer formulation.Overlay histogram showing Jurkat cells stained with ab32039 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32039, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (1µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

      • Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)
        Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab157243? Please let us know so that we can cite the reference in this datasheet.

      ab157243 has not yet been referenced specifically in any publications.

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