Recombinant Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)
![Western blot - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)](https://www.abcam.com/ps/products/157/ab157243/Images/ab157243-405349-anti-itk-emt-antibody-y401-bsa-and-azide-free-western-blot.gif "Western blot - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y401] to ITK/EMT - BSA and Azide free
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-ITK/EMT antibody [Y401] - BSA and Azide free
See all ITK/EMT primary antibodies -
Description
Rabbit monoclonal [Y401] to ITK/EMT - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab157243 is the carrier-free version of ab32039 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab157243 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y401 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 72 kDa).
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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Flow Cyt
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 72 kDa). |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Plays a role in T-cell proliferation and differentiation. -
Tissue specificity
T-cell lines and natural killer cell lines. -
Involvement in disease
Defects in ITK are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1) [MIM:613011]. LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
Cellular localization
Cell membrane. Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells. - Information by UniProt
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Database links
- Entrez Gene: 3702 Human
- Omim: 186973 Human
- SwissProt: Q08881 Human
- Unigene: 558348 Human
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Alternative names
- EMT antibody
- Homolog of mouse T cell itk/tsk antibody
- IL 2 inducible T cell kinase antibody
see all
Images
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Western blot - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)Anti-ITK/EMT antibody [Y401] (ab32039) at 1/5000 dilution + Jurkat cell lysate
Predicted band size: 72 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?This data was developed using ab32039, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)](https://www.abcam.com/ps/products/157/ab157243/Images/ab157243-2-anti-itk-emt-antibody-y401-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)This data was developed using ab32039, the same antibody clone in a different buffer formulation.ab32039 at a 1/250 dilution, staining ITK/EMT in paraffin embedded human lymph node tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Flow Cytometry - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)](https://www.abcam.com/ps/products/157/ab157243/Images/ab157243-1-anti-itk-emt-antibody-y401-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)This data was developed using ab32039, the same antibody clone in a different buffer formulation.Overlay histogram showing Jurkat cells stained with ab32039 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32039, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (1µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions. -
![Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)](https://www.abcam.com/ps/products/157/ab157243/Images/ab157243-4-benefits-of-recombinant-antibodies.png)
Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab157243 has not yet been referenced specifically in any publications.