Recombinant Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y401] to ITK/EMT - BSA and Azide free
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ITK/EMT antibody [Y401] - BSA and Azide free
See all ITK/EMT primary antibodies -
Description
Rabbit monoclonal [Y401] to ITK/EMT - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat whole cell lysate and MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate. Flow Cyt (Intra): Jurkat cells.
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General notes
ab157243 is the carrier-free version of ab32039.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y401 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- PE Anti-ITK / EMT antibody [Y401] (ab305469)
- Alexa Fluor® 488 Anti-ITK / EMT antibody [Y401] (ab309864)
- Alexa Fluor® 647 Anti-ITK / EMT antibody [Y401] (ab310231)
- Alexa Fluor® 594 Anti-ITK / EMT antibody [Y401] (ab310681)
- Alexa Fluor® 555 Anti-ITK / EMT antibody [Y401] (ab312211)
- Alexa Fluor® 568 Anti-ITK / EMT antibody [Y401] (ab312700)
- Anti-ITK/EMT antibody [Y401] (ab32039)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 72 kDa).
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 72 kDa). |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
Target
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Function
Plays a role in T-cell proliferation and differentiation. -
Tissue specificity
T-cell lines and natural killer cell lines. -
Involvement in disease
Defects in ITK are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1) [MIM:613011]. LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
Cellular localization
Cell membrane. Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells. - Information by UniProt
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Database links
- Entrez Gene: 3702 Human
- Omim: 186973 Human
- SwissProt: Q08881 Human
- Unigene: 558348 Human
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Alternative names
- EMT antibody
- Homolog of mouse T cell itk/tsk antibody
- IL 2 inducible T cell kinase antibody
see all
Images
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This data was developed using ab32039, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling ITK/EMT with purified ab320396 at 1:50 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as a isotype control. Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
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All lanes : Anti-ITK/EMT antibody [Y401] (ab32039) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?This data was developed using ab32039, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
ab181602 was used as GAPDH loading control.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab157243 has not yet been referenced specifically in any publications.