Recombinant
RabMAb

Recombinant Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20311] to ITLN1 + ITLN2
  • Suitable for: WB, IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ITLN1 + ITLN2 antibody [EPR20311]
    See all ITLN1+ITLN2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20311] to ITLN1 + ITLN2

  • Host species

    Rabbit

  • Specificity

    ITLN1 mRNA expression in stomach has been reported [PMID: 19549487]. Another report [PMID: 22768319] shows no staining on stomach at protein level. In our hands this antibody showed weak cytoplasmic staining on stomach samples.

  • Tested applications

    Suitable for: WB, IHC-Fr, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human ITLN1 aa 1-300. The exact sequence is proprietary.
    Database link: Q8WWA0

  • Positive control

    • WB: Human ITLN1 DDDDK tagged recombinant protein (aa17-313); Human ITLN2 His-tagged recombinant protein (aa27-325); Human, mouse and rat colon and small intestine lysates. IHC-P: Human, mouse and rat colon tissues. IHC-Fr: Mouse colon tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab210483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
IHC-Fr 1/500.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

Images

  • Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    All lanes : Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483) at 1/1000 dilution

    Lane 1 : Recombinant human ITLN1 protein (ab109151)
    Lane 2 : Human ITLN2 His-tagged recombinant protein (aa27-325)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa
    Observed band size: 36,37 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Frozen sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    Immunohistochemistry (Frozen sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling ITLN1 + ITLN2 with ab210483 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Cytoplasmic staining on goblet cells of mouse colon is observed [PMID: 22768319] [PMID: 19549487].

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling ITLN1 + ITLN2 with ab210483 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on goblet cells of human colon is observed [PMID: 22768319] [PMID: 19549487].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)

    Immunohistochemical analysis of paraffin-embedded  mouse colon tissue labeling ITLN1 + ITLN2 with ab210483 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on goblet cells of mouse colon is observed [PMID: 22768319] [PMID: 19549487].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)

    Immunohistochemical analysis of paraffin-embedded  rat colon tissue labeling ITLN1 + ITLN2 with ab210483 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on goblet cells of rat colon is observed [PMID: 22768319] [PMID: 19549487].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    All lanes : Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483) at 1/5000 dilution

    Lane 1 : Human colon lysate
    Lane 2 : Human fetal spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Spleen is a negative control (PMID: 11181563).

  • Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    All lanes : Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483) at 1/5000 dilution

    Lane 1 : Mouse colon lysate
    Lane 2 : Rat small intestine lysate
    Lane 3 : Mouse small intestine lysate
    Lane 4 : Rat colon lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/2: 1 minute; Lane 3/4: 30 seconds.

    The observed molecular weight is consistent with the literature (PMID: 11313366, PMID: 20628387).

  • Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483) at 1/5000 dilution + Human small intestine lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed molecular weight is consistent with the literature (PMID: 11313366, PMID: 20628387).

References (0)

Publishing research using ab210483? Please let us know so that we can cite the reference in this datasheet.

ab210483 has not yet been referenced specifically in any publications.

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