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    itln1--itln2-antibody-epr20311-ab210483.pdf

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RecombinantRabMAb

Recombinant Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question References (1)

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Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
  • Immunohistochemistry (Frozen sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
  • Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
  • Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
  • Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
  • Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20311] to ITLN1 + ITLN2
  • Suitable for: WB, IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Human

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Anti-ITLN1 antibody [EPR22488-27] (ab252927)

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Overview

  • Product name

    Anti-ITLN1 + ITLN2 antibody [EPR20311]
    See all ITLN1+ITLN2 primary antibodies
  • Description

    Rabbit monoclonal [EPR20311] to ITLN1 + ITLN2
  • Host species

    Rabbit
  • Specificity

    ITLN1 mRNA expression in stomach has been reported [PMID: 19549487]. Another report [PMID: 22768319] shows no staining on stomach at protein level. In our hands this antibody showed weak cytoplasmic staining on stomach samples.
  • Tested applications

    Suitable for: WB, IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human ITLN1 DDDDK tagged recombinant protein (aa17-313); Human ITLN2 His-tagged recombinant protein (aa27-325); Human, mouse and rat colon and small intestine lysates. IHC-P: Human, mouse and rat colon tissues. IHC-Fr: Mouse colon tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20311
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Other
    • Cardiovascular
    • Lipids / Lipoproteins
    • Adipose Related
    • Acrp
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Microbiology
    • Interspecies Interaction
    • Host Immune Response

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Related Products

    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab210483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
IHC-Fr
1/500.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).

IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
IHC-Fr
1/500.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).

IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Cellular localization

    ITLN1: Cell membrane. Secreted. Enriched in lipid rafts. ITLN2: Secreted.
  • Database links

    • Entrez Gene: 142683 Human
    • Entrez Gene: 55600 Human
    • Entrez Gene: 16429 Mouse
    • Entrez Gene: 493583 Mouse
    • Entrez Gene: 498284 Rat
    • Omim: 609873 Human
    • Omim: 609874 Human
    • SwissProt: Q8WWA0 Human
    • SwissProt: Q8WWU7 Human
    • SwissProt: O88310 Mouse
    • SwissProt: Q80ZA0 Mouse
    • Unigene: 385631 Human
    • Unigene: 50813 Human
    see all

Images

  • Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    All lanes : Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483) at 1/1000 dilution

    Lane 1 : Recombinant human ITLN1 protein (ab109151)
    Lane 2 : Human ITLN2 His-tagged recombinant protein (aa27-325)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa
    Observed band size: 36,37 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Frozen sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    Immunohistochemistry (Frozen sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling ITLN1 + ITLN2 with ab210483 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Cytoplasmic staining on goblet cells of mouse colon is observed [PMID: 22768319] [PMID: 19549487].

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling ITLN1 + ITLN2 with ab210483 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on goblet cells of human colon is observed [PMID: 22768319] [PMID: 19549487].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)

    Immunohistochemical analysis of paraffin-embedded  mouse colon tissue labeling ITLN1 + ITLN2 with ab210483 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on goblet cells of mouse colon is observed [PMID: 22768319] [PMID: 19549487].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)

    Immunohistochemical analysis of paraffin-embedded  rat colon tissue labeling ITLN1 + ITLN2 with ab210483 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on goblet cells of rat colon is observed [PMID: 22768319] [PMID: 19549487].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    All lanes : Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483) at 1/5000 dilution

    Lane 1 : Human colon lysate
    Lane 2 : Human fetal spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Spleen is a negative control (PMID: 11181563).

  • Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    All lanes : Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483) at 1/5000 dilution

    Lane 1 : Mouse colon lysate
    Lane 2 : Rat small intestine lysate
    Lane 3 : Mouse small intestine lysate
    Lane 4 : Rat colon lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/2: 1 minute; Lane 3/4: 30 seconds.

    The observed molecular weight is consistent with the literature (PMID: 11313366, PMID: 20628387).

  • Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483) at 1/5000 dilution + Human small intestine lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed molecular weight is consistent with the literature (PMID: 11313366, PMID: 20628387).

  • Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)
    Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (1)

Publishing research using ab210483? Please let us know so that we can cite the reference in this datasheet.

ab210483 has been referenced in 1 publication.

  • Porter CM  et al. Lactoferrin CpG Island Hypermethylation and Decoupling of mRNA and Protein Expression in the Early Stages of Prostate Carcinogenesis. Am J Pathol 189:2311-2322 (2019). PubMed: 31499027

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