Overview

  • Product name

    Anti-ITM2A antibody
  • Description

    Rabbit polyclonal to ITM2A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human ITM2A aa 73-123 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    CIYKYFMP KSTIYRGEMC FFDSEDPANS LRGGEPNFLP VTEEADIRED DNI


    Database link: O43736

  • Positive control

    • Rat brain vascular endothelial cells (RBE4); Rat brain tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab203620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

When using a fluorescent probe the recommended dilution is 1/50 – 1/200.

Target

Images

  • Immunofluorescent analysis of 4% PFA fixed rat brain vascular endothelial cells (RBE4) labeling ITM2A with ab203620 at 1/100 dilution, followed by conjugated secondary antibody(green) and DAPI staining (blue). 

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling ITM2A with ab203620 at 1/200 dilution overnight at 4°C followed by Goat Anti-Rabbit IgG, Cy3 at 1/200 dilution for 40 minutes at 37°C.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat brain tissue labeling ITM2A with ab203620 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

References

ab203620 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab203620.
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