Overview

  • Product name

  • Description

    Goat polyclonal to ITM2B
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human, a wide range of other species
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    HDKETYKLQRRETIK

    with a Cysteine residue linker, corresponding to internal sequence amino acids 224-238 of Human ITM2B (NP_068839.1).

  • Positive control

    • Human Small Intestine (Myenteric Plexus) tissue; Human Brain (Cerebellum and Hippocampus) lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab129282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 30 kDa.
IHC-P Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Functions as a protease inhibitor. Plays a role in APP processing regulating the physiological production of the beta amyloid peptide. Restricts docking of gamma-secretase to APP and access of alpha- and beta-secretase to their cleavage APP sequence.
  • Tissue specificity

    Expressed in brain and in other tissues.
  • Involvement in disease

    Defects in ITM2B are a cause of cerebral amyloid angiopathy ITM2B-related type 1 (CAA-ITM2B1) [MIM:176500]. A disorder characterized by amyloid deposition in the walls of cerebral blood vessels and neurodegeneration in the central nervous system. Cerebral amyloid angiopathy, non-neuritic and perivascular plaques and neurofibrillary tangles are the predominant pathological lesions. Clinical features include progressive mental deterioration, spasticity and muscular rigidity.
    Defects in ITM2B are a cause of cerebral amyloid angiopathy ITM2B-related type 2 (CAA-ITM2B2) [MIM:117300]; also known as heredopathia ophthalmo-oto-encephalica. A disorder characterized by amyloid deposition in the walls of the blood vessels of the cerebrum, choroid plexus, cerebellum, spinal cord and retina. Plaques and neurofibrillary tangles are observed in the hippocampus. Clinical features include progressive ataxia, dementia, cataracts and deafness.
  • Sequence similarities

    Belongs to the ITM2 family.
    Contains 1 BRICHOS domain.
  • Post-translational
    modifications

    The C-terminal part of the ectodomain is processed by furin and related proteases producing a secreted peptide of 4 to 5 kDa. For the ABRI and ADAN variants the C-terminal secreted peptide is larger and may produce amyloid fibrils responsible for neuronal dysfunction and dementia. The remaining part of the ectodomain containing the BRICHOS domain is cleaved by ADAM10 and is secreted as a peptide of 25 kDa. The membrane-bound N-terminal fragment (NTF) of 22 kDa is further proteolytically processed by SPPL2A and SPPL2B through regulated intramembrane proteolysis producing a secreted peptide (BRI2C) and an intracellular domain (ICD) released in the cytosol.
  • Cellular localization

    Golgi apparatus membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ABRI antibody
    • ABri/ADan amyloid peptide antibody
    • BRI 2 antibody
    • BRI antibody
    • BRI2 antibody
    • BRICD 2B antibody
    • BRICD2B antibody
    • BRICHOS domain containing 2B antibody
    • E25B antibody
    • E3 16 antibody
    • E3-16 antibody
    • FBD antibody
    • imBRI2 antibody
    • Immature BRI2 antibody
    • Integral membrane protein 2B antibody
    • ITM 2B antibody
    • ITM2B antibody
    • ITM2B_HUMAN antibody
    • Protein E25B antibody
    • RDGCA antibody
    • Transmembrane protein BRI antibody
    see all

Images

  • Anti-ITM2B antibody (ab129282) at 1 µg/ml + Human Hippocampus lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 30 kDa

  • ab129282, at 3.75 µg/ml, staining ITM2B in Formalin-fixed, Paraffin-embedded Human Small Intestine (Myenteric Plexus) tissue by Immunohistochemistry.

References

ab129282 has not yet been referenced specifically in any publications.

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