Key features and details
- Mouse monoclonal [OTI1C11] to ITM2B
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-ITM2B antibody [OTI1C11]
See all ITM2B primary antibodies
DescriptionMouse monoclonal [OTI1C11] to ITM2B
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Recombinant full length Human ITM2B produced in HEK293T cells (NP_068839).
- HEK293T cell lysate transfected with pCMV6-ENTRY ITM2B cDNA; Human kidney tissue; HeLa and Jurkat cells.
Dilute in PBS (pH7.3) before use.
The clone number has been updated from 1C11 to OTI1C11, both clone numbers name the same clone.
This product was changed from ascites to tissue culture supernatant on 5th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol, PBS
Concentration information loading...
PurityTissue culture supernatant
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab119044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 30 kDa.|
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionFunctions as a protease inhibitor. Plays a role in APP processing regulating the physiological production of the beta amyloid peptide. Restricts docking of gamma-secretase to APP and access of alpha- and beta-secretase to their cleavage APP sequence.
Tissue specificityExpressed in brain and in other tissues.
Involvement in diseaseDefects in ITM2B are a cause of cerebral amyloid angiopathy ITM2B-related type 1 (CAA-ITM2B1) [MIM:176500]. A disorder characterized by amyloid deposition in the walls of cerebral blood vessels and neurodegeneration in the central nervous system. Cerebral amyloid angiopathy, non-neuritic and perivascular plaques and neurofibrillary tangles are the predominant pathological lesions. Clinical features include progressive mental deterioration, spasticity and muscular rigidity.
Defects in ITM2B are a cause of cerebral amyloid angiopathy ITM2B-related type 2 (CAA-ITM2B2) [MIM:117300]; also known as heredopathia ophthalmo-oto-encephalica. A disorder characterized by amyloid deposition in the walls of the blood vessels of the cerebrum, choroid plexus, cerebellum, spinal cord and retina. Plaques and neurofibrillary tangles are observed in the hippocampus. Clinical features include progressive ataxia, dementia, cataracts and deafness.
Sequence similaritiesBelongs to the ITM2 family.
Contains 1 BRICHOS domain.
modificationsThe C-terminal part of the ectodomain is processed by furin and related proteases producing a secreted peptide of 4 to 5 kDa. For the ABRI and ADAN variants the C-terminal secreted peptide is larger and may produce amyloid fibrils responsible for neuronal dysfunction and dementia. The remaining part of the ectodomain containing the BRICHOS domain is cleaved by ADAM10 and is secreted as a peptide of 25 kDa. The membrane-bound N-terminal fragment (NTF) of 22 kDa is further proteolytically processed by SPPL2A and SPPL2B through regulated intramembrane proteolysis producing a secreted peptide (BRI2C) and an intracellular domain (ICD) released in the cytosol.
Cellular localizationGolgi apparatus membrane. Cell membrane.
- Information by UniProt
- ABRI antibody
- ABri/ADan amyloid peptide antibody
- BRI 2 antibody
All lanes : Anti-ITM2B antibody [OTI1C11] (ab119044) at 1/2000 dilution
Lane 1 : HEK293T lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T lysate transfected with pCMV6-ENTRY ITM2B cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 30 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202377)
ab119044, at 1/150 dilution, staining ITM2B in paraffin embedded Human kidney by Immunohistochemistry.
ab119044 at 1/100 dilution staining ITM2B in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
ab119044 at 1/100 dilution staining ITM2B in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
ab119044 has not yet been referenced specifically in any publications.