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  1. Link

    itpa-antibody-epr8780-ab134937.pdf

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Signal Transduction Metabolism Amino Acids
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-ITPA antibody [EPR8780] (ab134937)

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Western blot - Anti-ITPA antibody [EPR8780] (ab134937)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPA antibody [EPR8780] (ab134937)
  • Western blot - Anti-ITPA antibody [EPR8780] (ab134937)
  • Anti-ITPA antibody [EPR8780] (ab134937)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8780] to ITPA
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human

You may also be interested in

Knockout
Product image
Human ITPA knockout HEK293T cell line (ab266679)

View more associated products

Overview

  • Product name

    Anti-ITPA antibody [EPR8780]
    See all ITPA primary antibodies
  • Description

    Rabbit monoclonal [EPR8780] to ITPA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ITPA aa 100-200. The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human breast carcinoma tissue. WB: HeLa, HepG2, Jurkat and K562 cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Inosine triphosphate pyrophosphatase

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8780
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Associated products

  • Alternative Versions

    • Anti-ITPA antibody [EPR8780] - BSA and Azide free (ab248742)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human ITPA knockout HEK293T cell line (ab266679)
  • KO cell lysates

    • Human ITPA knockout HEK293T cell lysate (ab258477)
  • Positive Controls

    • HepG2 cytoplasmic extract lysate (ab14659)
    • Jurkat cytoplasmic extract lysate (ab14842)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab7899)
    • K562 whole cell lysate (ab7911)

Applications

Our Abpromise guarantee covers the use of ab134937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For antigen retrieval: heat up to 98°C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended.
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Hydrolyzes ITP and dITP to their respective monophosphate derivatives. Xanthosine 5'-triphosphate (XTP) is also a potential substrate. May be the major enzyme responsible for regulating ITP concentration in cells.
    • Tissue specificity

      Ubiquitous. Highly expressed in heart, liver, sex glands, thyroid and adrenal gland.
    • Involvement in disease

      Defects in ITPA are the cause of inosine triphosphate pyrophosphohydrolase deficiency (ITPA deficiency) [MIM:147520]. It is a common inherited trait characterized by the abnormal accumulation of inosine triphosphate (ITP) in erythrocytes and also leukocytes and fibroblasts. The pathological consequences of ITPA deficiency, if any, are unknown. However, it might have pharmacogenomic implications and be related to increased drug toxicity of purine analog drugs. Three different human populations have been reported with respect to their ITPase activity: high, mean (25% of high) and low activity. The variant Thr-32 is associated with complete loss of enzyme activity, may be by altering the local secondary structure of the protein. Heterozygotes for this polymorphism have 22.5% of the control activity: this is consistent with a dimeric structure of the enzyme.
    • Sequence similarities

      Belongs to the HAM1 NTPase family.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession Q9BY32 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3704 Human
      • Omim: 147520 Human
      • SwissProt: Q9BY32 Human
      • Unigene: 415299 Human
      • Alternative names

        • C20orf37 antibody
        • dJ794I6.3 antibody
        • HLC14-06-P antibody
        • Inosine triphosphatase (nucleoside triphosphate pyrophosphatase) antibody
        • Inosine triphosphatase antibody
        • inosine triphosphatase-A antibody
        • Inosine triphosphate pyrophosphatase antibody
        • Inosine triphosphate pyrophosphohydrolase antibody
        • Itpa antibody
        • ITPA_HUMAN antibody
        • ITPase antibody
        • My049 antibody
        • My049 protein antibody
        • Non canonical purine NTP pyrophosphatase antibody
        • Non standard purine NTP pyrophosphatase antibody
        • NTPase antibody
        • nucleoside triphosphate diphosphatase antibody
        • Nucleoside triphosphate pyrophosphatase antibody
        • OK/SW-cl.9 antibody
        • OTTHUMP00000030094 antibody
        • OTTHUMP00000160459 antibody
        • Putative oncogene protein hlc14-06-p antibody
        see all

      Images

      • Western blot - Anti-ITPA antibody [EPR8780] (ab134937)
        Western blot - Anti-ITPA antibody [EPR8780] (ab134937)
        All lanes : Anti-ITPA antibody [EPR8780] (ab134937) at 1/1000 dilution

        Lane 1 : Wild-type HEK293T cell lysate
        Lane 2 : ITPA knockout HEK293T cell lysate
        Lane 3 : Jurkat cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

        Predicted band size: 21 kDa
        Observed band size: 21 kDa



        Lanes 1-3: Merged signal (red and green). Green - ab134937 observed at 21 kDa. Red - loading control ab8245 observed at 36 kDa.

         ab134937 Anti-ITPA antibody [EPR8780]  was shown to specifically react with ITPA in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266679 (knockout cell lysate ab258477) was used. Wild-type and ITPA knockout samples were subjected to SDS-PAGE. ab134937 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPA antibody [EPR8780] (ab134937)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPA antibody [EPR8780] (ab134937)

        Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human breast carcinoma tissue, labelling ITPA using ab134937 at a 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Western blot - Anti-ITPA antibody [EPR8780] (ab134937)
        Western blot - Anti-ITPA antibody [EPR8780] (ab134937)
        All lanes : Anti-ITPA antibody [EPR8780] (ab134937) at 1/1000 dilution

        Lane 1 : Jurkat cell lysate
        Lane 2 : HepG2 cell lysate
        Lane 3 : HeLa cell lysate
        Lane 4 : K562 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

        Developed using the ECL technique.

        Predicted band size: 21 kDa

      • Anti-ITPA antibody [EPR8780] (ab134937)
        Anti-ITPA antibody [EPR8780] (ab134937)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab134937? Please let us know so that we can cite the reference in this datasheet.

      ab134937 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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