Overview

  • Product name

  • Description

    Rabbit polyclonal to ITPK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 171-405 of Human ITPK1 (AAH18192)

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji and HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 46 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway. Also acts as an inositol polyphosphate phosphatase that dephosphorylate Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3, and Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4. May also act as an isomerase that interconverts the inositol tetraphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium. Probably acts as the rate-limiting enzyme of the InsP6 pathway. Modifies TNF-alpha-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain.
  • Tissue specificity

    Expressed in brain > heart > skeletal muscle = kidney = pancreas = liver = placenta > lung. In brain, it is expressed in cerebellum, cerebral cortex, medulla, spinal cord, occipital lobe, frontal lobe, temporal lobe and putamen.
  • Sequence similarities

    Belongs to the ITPK1 family.
    Contains 1 ATP-grasp domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3 antibody
    • 4)P(3) 5/6-kinase antibody
    • 4-trisphosphate 5/6-kinase antibody
    • Inositol 1 3 4 triphosphate 5/6 kinase antibody
    • Inositol 1 3 4 trisphosphate 5/6 kinase antibody
    • Inositol 1 antibody
    • Inositol tetrakisphosphate 1 kinase antibody
    • Inositol triphosphate 5/6 kinase antibody
    • Inositol-tetrakisphosphate 1-kinase antibody
    • Inositol-triphosphate 5/6-kinase antibody
    • Ins(1 3 4)P(3) 5/6 kinase antibody
    • Ins(1 antibody
    • ITPK 1 antibody
    • itpk1 antibody
    • ITPK1_HUMAN antibody
    • ITRPK 1 antibody
    • ITRPK1 antibody
    see all

Images

  • Anti-ITPK1 antibody (ab97447) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 46 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97447 at 1/200 dilution (1) and merged with DNA probe (2).

References

ab97447 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab97447.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up