Anti-ITPK1 antibody [EPR10631] (ab175222)
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- Protocols
Overview
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Product nameAnti-ITPK1 antibody [EPR10631]
See all ITPK1 primary antibodies -
DescriptionRabbit monoclonal [EPR10631] to ITPK1
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WB, IP, Flow Cytmore details
Unsuitable for: ICC -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ITPK1 aa 350 to the C-terminus. The exact sequence is proprietary.
Database link: Q13572 -
Positive control
- Fetal brain, fetal heart, HepG2 and Hela lysates; Human brain and liver tissues; Hela cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR10631
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab175222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/100. | |
| WB | 1/10000 - 1/50000. Predicted molecular weight: 46 kDa. | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionKinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway. Also acts as an inositol polyphosphate phosphatase that dephosphorylate Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3, and Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4. May also act as an isomerase that interconverts the inositol tetraphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium. Probably acts as the rate-limiting enzyme of the InsP6 pathway. Modifies TNF-alpha-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain.
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Tissue specificityExpressed in brain > heart > skeletal muscle = kidney = pancreas = liver = placenta > lung. In brain, it is expressed in cerebellum, cerebral cortex, medulla, spinal cord, occipital lobe, frontal lobe, temporal lobe and putamen.
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Sequence similaritiesBelongs to the ITPK1 family.
Contains 1 ATP-grasp domain. - Information by UniProt
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Database links
- Entrez Gene: 3705 Human
- Omim: 601838 Human
- SwissProt: Q13572 Human
- Unigene: 308122 Human
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Alternative names
- 3 antibody
- 4)P(3) 5/6-kinase antibody
- 4-trisphosphate 5/6-kinase antibody
see all
Images
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![Immunoprecipitation - Anti-ITPK1 antibody [EPR10631] (ab175222)](https://www.abcam.com/ps/products/175/ab175222/Images/ab175222-201592-ip1.jpg)
Immunoprecipitation - Anti-ITPK1 antibody [EPR10631] (ab175222)Western blot analysis on immunoprecipitation pellet from (1) HeLa cells lysate or (2) 1X PBS (negative control) using ab175222 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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![Flow Cytometry - Anti-ITPK1 antibody [EPR10631] (ab175222)](https://www.abcam.com/ps/products/175/ab175222/Images/ab175222-201590-flow.jpg)
Flow Cytometry - Anti-ITPK1 antibody [EPR10631] (ab175222)Flow cytometric analysis of permeabilized HeLa cells labeling ITPK1 with ab175222 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPK1 antibody [EPR10631] (ab175222)](https://www.abcam.com/ps/products/175/ab175222/Images/ab175222-201588-ab175222ihc2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPK1 antibody [EPR10631] (ab175222)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ITPK1 with ab175222 at 1/50 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPK1 antibody [EPR10631] (ab175222)](https://www.abcam.com/ps/products/175/ab175222/Images/ab175222-201586-ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPK1 antibody [EPR10631] (ab175222)Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ITPK1 with ab175222 at 1/50 dilution.
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![Western blot - Anti-ITPK1 antibody [EPR10631] (ab175222)](https://www.abcam.com/ps/products/175/ab175222/Images/ab175222-201585-wb1.jpg)
Western blot - Anti-ITPK1 antibody [EPR10631] (ab175222)All lanes : Anti-ITPK1 antibody [EPR10631] (ab175222) at 1/10000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Fetal liver lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Hela cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 46 kDa
Protocols
References
ab175222 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
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