RecombinantRabMAb

Recombinant Anti-ITPK1 antibody [EPR10631] (ab175222)

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Immunoprecipitation - Anti-ITPK1 antibody [EPR10631] (ab175222)
  • Flow Cytometry - Anti-ITPK1 antibody [EPR10631] (ab175222)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPK1 antibody [EPR10631] (ab175222)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPK1 antibody [EPR10631] (ab175222)
  • Western blot - Anti-ITPK1 antibody [EPR10631] (ab175222)
  • Anti-ITPK1 antibody [EPR10631] (ab175222)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10631] to ITPK1
  • Suitable for: IHC-P, WB, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-ITPK1 antibody [EPR10631]
    See all ITPK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10631] to ITPK1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IP, Flow Cytmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ITPK1 aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: Q13572

  • Positive control

    • Fetal brain, fetal heart, HepG2 and Hela lysates; Human brain and liver tissues; Hela cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10631

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/10000 - 1/50000. Predicted molecular weight: 46 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC.

    • Target

    • Function

      Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway. Also acts as an inositol polyphosphate phosphatase that dephosphorylate Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3, and Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4. May also act as an isomerase that interconverts the inositol tetraphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium. Probably acts as the rate-limiting enzyme of the InsP6 pathway. Modifies TNF-alpha-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain.

    • Tissue specificity

      Expressed in brain > heart > skeletal muscle = kidney = pancreas = liver = placenta > lung. In brain, it is expressed in cerebellum, cerebral cortex, medulla, spinal cord, occipital lobe, frontal lobe, temporal lobe and putamen.

    • Sequence similarities

      Belongs to the ITPK1 family.
      Contains 1 ATP-grasp domain.
    • Information by UniProt

    • Database links

      • Alternative names

        • 3 antibody
        • 4)P(3) 5/6-kinase antibody
        • 4-trisphosphate 5/6-kinase antibody
        • Inositol 1 3 4 triphosphate 5/6 kinase antibody
        • Inositol 1 3 4 trisphosphate 5/6 kinase antibody
        • Inositol 1 antibody
        • Inositol tetrakisphosphate 1 kinase antibody
        • Inositol triphosphate 5/6 kinase antibody
        • Inositol-tetrakisphosphate 1-kinase antibody
        • Inositol-triphosphate 5/6-kinase antibody
        • Ins(1 3 4)P(3) 5/6 kinase antibody
        • Ins(1 antibody
        • ITPK 1 antibody
        • itpk1 antibody
        • ITPK1_HUMAN antibody
        • ITRPK 1 antibody
        • ITRPK1 antibody
        see all

      Images

      • Immunoprecipitation - Anti-ITPK1 antibody [EPR10631] (ab175222)
        Immunoprecipitation - Anti-ITPK1 antibody [EPR10631] (ab175222)

        Western blot analysis on immunoprecipitation pellet from (1) HeLa cells lysate or (2) 1X PBS (negative control) using ab175222 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

      • Flow Cytometry - Anti-ITPK1 antibody [EPR10631] (ab175222)
        Flow Cytometry - Anti-ITPK1 antibody [EPR10631] (ab175222)

        Flow cytometric analysis of permeabilized HeLa cells labeling ITPK1 with ab175222 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPK1 antibody [EPR10631] (ab175222)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPK1 antibody [EPR10631] (ab175222)

        Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ITPK1 with ab175222 at 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPK1 antibody [EPR10631] (ab175222)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPK1 antibody [EPR10631] (ab175222)

        Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ITPK1 with ab175222 at 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Western blot - Anti-ITPK1 antibody [EPR10631] (ab175222)
        Western blot - Anti-ITPK1 antibody [EPR10631] (ab175222)
        All lanes : Anti-ITPK1 antibody [EPR10631] (ab175222) at 1/10000 dilution

        Lane 1 : Fetal brain lysate
        Lane 2 : Fetal liver lysate
        Lane 3 : HepG2 cell lysate
        Lane 4 : Hela cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 46 kDa

      • Anti-ITPK1 antibody [EPR10631] (ab175222)
        Anti-ITPK1 antibody [EPR10631] (ab175222)

      References (1)

      Publishing research using ab175222? Please let us know so that we can cite the reference in this datasheet.

      ab175222 has been referenced in 1 publication.

      • Desfougères Y  et al. ITPK1 mediates the lipid-independent synthesis of inositol phosphates controlled by metabolism. Proc Natl Acad Sci U S A 116:24551-24561 (2019). PubMed: 31754032

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