Product nameAnti-ITPR3 antibody
See all ITPR3 primary antibodies
DescriptionRabbit polyclonal to ITPR3
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human ITPR3 aa 1755-1825. The exact sequence is proprietary. NP_002215.2
Database link: Q14573
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 304 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionReceptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium.
Tissue specificityExpressed in intestinal crypt and villus epithelial cells.
Sequence similaritiesBelongs to the InsP3 receptor family.
Contains 5 MIR domains.
DomainThe receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.
modificationsPhosphorylated on tyrosine residues. Phosphorylated by AKT1 on serine and/or threonine residues.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- 4 antibody
- 5-trisphosphate receptor antibody
- 5-trisphosphate receptor type 3 antibody
All lanes : Anti-ITPR3 antibody (ab264283) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 304 kDa
Exposure time: 30 seconds
ITPR3 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264283 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264283 at 1 µg/ml.
Lane 1: ab264283 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 10 secs.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264283 has not yet been referenced specifically in any publications.